In vitro and in vivo assessment of chitosan modified Urocanic acid (cas 104-98-3) as gene carrier

Add time:08/14/2019    Source:sciencedirect.com

Chitosan nanoparticles modified with 10 and 30% Urocanic acid (cas 104-98-3) (CUA) via carbodiimide crosslinking were examined for an efficient gene delivery carrier. The CUA gene carrier was characterized by FTIR, TEM, SEM and the in vitro transfection efficiency CUA polyplex was tested with HeLa and 3T3 cells. The loading efficiency of CUA complexes with DNA was assessed at different N/P ratio of 1, 2, 4, 6, 8, and 10. The DNA loading efficiency was found be to > 85% for chitosan, CUA10 and CUA30% and the DNA protection ability of CUA10 and CUA30 nanoparticle complexes was confirmed upon incubation with NheI and HindIII. The cell toxicity and cell viability results have supported the non-toxic nature of CUA10 and CUA30 nanoparticles. In vitro transfection efficiency of CUA10 and CUA30 polyplex was tested for EGFP expression in 3T3 and HeLa cells and a relative maximum % transfection of about 10% was confirmed by CUA10 and CUA30 after 96 h transfection. The feasibility and biocompatibility of CUA gene carrier in transgenic chickens was also demonstrated. The in vitro transfection and in vivo embryonic viability studies further confirmed the CUA as promising gene carrier because of the improved biocompatibility and DNA protection ability.

We also recommend Trading Suppliers and Manufacturers of Urocanic acid (cas 104-98-3). Pls Click Website Link as below: cas 104-98-3 suppliers

Prev:Development of an analytical method for Urocanic acid (cas 104-98-3) isomers in fish based on reactive extraction cleanup and chaotropic chromatography techniques
Next:Nonadiabatic dynamics simulations of photoexcited Urocanic acid (cas 104-98-3))