Preparation of MALTOTRIOSE (cas 1109-28-0) from fermentation broth by hydrolysis of pullulan using pullulanase

Add time:08/13/2019    Source:sciencedirect.com

In this study, we prepared MALTOTRIOSE (cas 1109-28-0) from the fermentation broth of Auerobasidium Pullulans CJ001 isolated from the sea mud by hydrolysis of pullulan with pullulanase. The fermentation broth was centrifuged to remove the microorganisms and then hydrolysed by pullulanase. The optimal hydrolysis conditions were obtained as follows: time, 9.40 h; pH, 4.92; temperature, 47.88 °C; pullulanase, 10 ASPU/g. Under these optimum hydrolysis conditions, the maximum dextrose equivalent value reached 31.86. The hydrolysates were filtrated through a filter membrane to separate any particle with molecular weight higher than 1000 Da, concentrated to ∼20%, and precipitated with 8 volumes of absolute ethanol. The precipitate was dried at 80 °C for 2 h to yield the maltotriose product. The maltotriose content in the product and the yield of maltotriose were 92.13% and 90.23%, respectively. The results indicate that this was a promising way of maltotriose production.

We also recommend Trading Suppliers and Manufacturers of MALTOTRIOSE (cas 1109-28-0). Pls Click Website Link as below: cas 1109-28-0 suppliers

Prev:Infrared spectra-structure correlations of carbamic acid: aryl-, alkyl esters
Next:High-level expression of soluble recombinant proteins in Escherichia coli using an HE-MALTOTRIOSE (cas 1109-28-0)-binding protein fusion tag)