The interaction of l-ascorbic acid with the active center of myrosinase

Add time:08/20/2019    Source:sciencedirect.com

Only l-ascorbic acid activated plant myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1), whereas ascorbic acid analogs did not.The enzyme protein was conformationally changed by the addition of l-ascorbic acid to the spectrophotometric analysis, approx. 1.5 amino residues appeared on the surface of the enzyme and about 2.3 tryptophan residues were buried in the molecule when 1 mM l-ascorbic acid was added.Optimum temperature for the myrosine activity was approx. 55°C without l-ascorbic acid, but with l-ascorbic acid it was about 35°C; that for β-glucosidase activity was the same (55°C) with or without l-ascorbic acid.The effect of chemical modification of the functional groups of myrosinase on the interaction of l-ascorbic acid was investigated and the interaction of l-ascorbic acid with the active center of the enzyme is proposed.

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