ArticleDissecting the Ribosomal Inhibition Mechanisms of Edeine (cas 11006-90-9) and Pactamycin: The Universally Conserved Residues G693 and C795 Regulate P-Site RNA Binding

Add time:08/15/2019    Source:sciencedirect.com

The crystal structures of the universal translation-initiation inhibitors Edeine (cas 11006-90-9) and pactamycin bound to ribosomal 30S subunit have revealed that edeine induces base pairing of G693:C795, residues that constitute the pactamycin binding site. Here, we show that base pair formation by addition of edeine inhibits tRNA binding to the P site by preventing codon-anticodon interaction and that addition of pactamycin, which rebreaks the base pair, can relieve this inhibition. In addition, edeine induces translational misreading in the A site, at levels comparable to those induced by the classic misreading antibiotic streptomycin. Binding of pactamycin between residues G693 and C795 strongly inhibits translocation with a surprising tRNA specificity but has no effect on translation initiation, suggesting that reclassification of this antibiotic is necessary. Collectively, these results suggest that the universally conserved G693:C795 residues regulate tRNA binding at the P site of the ribosome and influence translocation efficiency.

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