Sandwich-type amperometric immunosensor for cancer biomarker based on signal amplification strategy of multiple enzyme-linked antibodies as probes modified with carbon nanotubes and concanavalin A

Add time:08/21/2019    Source:sciencedirect.com

A sandwich-type amperometric immunosensor for carcinoembryonic antigen was fabricated by using functionalized carbon nanotubes decorated with concanavalin A along with horseradish peroxidase-labeled carcinoembryonic secondary antibodies. The HRP-Ab2 was assembled onto carbon nanotubes templates based on the biospecific affinity between concanavalin A and HRP. The carcinoembryonic antibody was immobilized on the gold electrode modified by monolayer cysteine. The response is linear in the range of 0.05–5 ng mL−1 and 5–200 ng mL−1, with a detection limit of 0.018 ng mL−1. The biosensor can be used in serum directly.

We also recommend Trading Suppliers and Manufacturers of CONCANAVALIN A TYPE III (cas 11028-71-0). Pls Click Website Link as below: cas 11028-71-0 suppliers

Prev:Electrochemical sensing of concanavalin A and ovalbumin interaction in solution
Next:Raft-dependent endocytic movement and intracellular cluster formation during T cell activation triggered by concanavalin A)