Isolation of carcinolipin (cas 19477-24-8) by combined liquid-solid and liquid-liquid chromatography

Add time:08/22/2019    Source:sciencedirect.com

The low polarity of Carcinolopin and its positive reaction to colour tests for sterols indicated that this substance might be a sterol ester. Cholesterol esters separated from beef liver lipids by silicic acid chromatography showed a stimulating effect on the incorporation of labelled algal protein hydrolysate into soluble RNA of rat liver in vitro. A group of cholesterol esters with saturated fatty acids was separated from cholesterol oleate and palmitoleate by elution with carbon tetrachloride from silicic acid columns. Only the saturated fraction had a protein synthesis-stimulating effect. A reversed-phase liquid-liquid chromatographic technique was developed for the fractionation of individual esters with saturated fatty acids. 10% Apiezon M grease on Celite 545 was used as stationary phase and the esters were eluted with acetone in methanol by an ascending technique. Only one fraction, containing a fatty acid with carbon number 16.7 as the main component, was active. It seems that carcinolipin (cas 19477-24-8) is an ester of cholesterol with a branced-chain fatty acid.

We also recommend Trading Suppliers and Manufacturers of carcinolipin (cas 19477-24-8). Pls Click Website Link as below: cas 19477-24-8 suppliers

Prev:A chromatographic method for the quantitative determination of cholesterol 14-methylhexadecanoate (carcinolipin (cas 19477-24-8)) in biological materials
Next:Purification of the fatty acid present in carcinolipin (cas 19477-24-8))