Short CommunicationCrystal structure of a biliverdin (cas 114-25-0)-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation
-
Add time:08/25/2019 Source:sciencedirect.com
Small, ultra-red fluorescence protein (smURFP) introduces the non-native biliverdin (BV) chromophore to phycobiliproteins (PBPs), allowing them to be used as transgenic labels for in vivo mammalian imaging. Presently, no structural information exists for PBPs bound to the non-native BV chromophore, which limits the further development of smURFP and related proteins as imaging labels or indicators. Here we describe the first crystal structure of a PBP bound to BV. The structures of smURFP-Y56R with BV and smURFP-Y56F without BV reveal unique oligomerization interfaces different from those in wild-type PBPs bound to native chromophores. Our structures suggest that the oligomerization interface affects the BV binding site, creating a link between oligomerization and chromophorylation that we confirmed through site-directed mutagenesis and that may help guide efforts to improve the notorious chromophorylation of smURFP and other PBPs engineered to bind BV.
We also recommend Trading Suppliers and Manufacturers of biliverdin (cas 114-25-0). Pls Click Website Link as below: cas 114-25-0 suppliers
Prev:ReviewRedox Functions of Heme Oxygenase-1 and biliverdin (cas 114-25-0) Reductase in Diabetes
Next:Reviewbiliverdin (cas 114-25-0) reductase isozymes in metabolism) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Singlet oxygen oxidation products of biliverdin (cas 114-25-0) IXα dimethyl ester08/30/2019
- Chiral recognition of bilirubin and biliverdin (cas 114-25-0) in liposomes and micelles08/29/2019
- Stabilization of structure in near-infrared fluorescent proteins by binding of biliverdin (cas 114-25-0) chromophore08/28/2019
- Reduced biliverdin (cas 114-25-0) reductase-A levels are associated with early alterations of insulin signaling in obesity08/27/2019
- Reviewbiliverdin (cas 114-25-0) reductase isozymes in metabolism08/26/2019
- ReviewRedox Functions of Heme Oxygenase-1 and biliverdin (cas 114-25-0) Reductase in Diabetes08/24/2019
- l-Histidine, arachidonic acid, biliverdin (cas 114-25-0), and l-cysteine-glutathione disulfide as potential biomarkers for cured pulmonary tuberculosis08/23/2019
- Is it worth expending energy to convert biliverdin (cas 114-25-0) into bilirubin?08/22/2019
- Research paperModeling structure and excitation of biliverdin (cas 114-25-0)-binding domains in infrared fluorescent proteins08/21/2019