Sequence analysis of the sulfated rhamno-oligosaccharides derived from a sulfated rhamnan (cas 111018-90-7)

Add time:08/29/2019    Source:sciencedirect.com

Three sulfated rhamno-oligosaccharides, designated O1, O2 and O3, were obtained by mild acid hydrolysis of the sulfated rhamnan and purified by gel-permeation chromatography. On the basis of one- and two-dimensional nuclear magnetic resonance (1D, 2D NMR) spectroscopic analyses, the oligosaccharide O1 was characterized to be α-l-Rhap-(2SO4)-(1 → 3)-α-l-Rhap. The fragmentation pattern of the homogeneous disaccharide in the product ion spectra was recognized by negative-ion electrospray tandem mass spectrometry with collision-induced dissociation (ES-CID MS/MS). With the principles established, the sequences of the oligosaccharides O2 and O3 were deduced to be α-l-Rhap-(2SO4)-(1 → 3)-α-l-Rhap-(1 → 3)-α-l-Rhap, and α-l-Rhap-(2SO4)-(1 → 3)-α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-α-l-Rhap (2SO4), respectively. The investigation demonstrated that the sulfated rhamnan-derived oligosaccharides were novel sulfated oligosaccharides different from those of other polysaccharides-degraded from algae, and it could be possible to determine the sequence of the sulfated rhamno-oligosaccharides directly from the glycosidic cleavage fragmentation in the product ion spectra.

We also recommend Trading Suppliers and Manufacturers of rhamnan (cas 111018-90-7). Pls Click Website Link as below: cas 111018-90-7 suppliers

Prev:rhamnan (cas 111018-90-7) sulfate from cell walls of monostroma latissimum
Next:Structure of rhamnan (cas 111018-90-7) sulfate from the green alga Monostroma nitidum and its anti-herpetic effect)