Detection of African horsesickness virus in infected spleens by a sandwich ELISA using two monoclonal antibodies specific for VP7
-
Add time:08/25/2019 Source:sciencedirect.com
A sandwich enzyme-linked immunosorbent assay (ELISA) for rapid detection of African horsesickness virus (AHSV) in infected spleens or cell culture supernatant was developed. This method uses two monoclonal antibodies (MAbs) which recognize two non-overlapping epitopes of the major core protein (VP7) to coat the solid phase, and one labeled with biotin as second antibody. This ELISA was evaluated for its ability to detect AHSV in infected spleens resulting in a sensitivity of 97.4% and a specificity of 100% compared with virus isolation in cell culture, and can be used for the detection of the nine different AHSV serotypes.
We also recommend Trading Suppliers and Manufacturers of VP7 major core antigen, African horsesickness virus (cas 138413-77-1). Pls Click Website Link as below: cas 138413-77-1 suppliers
Prev:Development of competitive ELISA for serodiagnosis on African horsesickness virus using baculovirus expressed VP7 and monoclonal antibody
Next:Expression and antigenic characterization of the major core protein VP7 of Chuzan virus, a member of the Palyam serogroup orbiviruses) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- African horsesickness: Pathogenesis and immunity09/01/2019
- Development of probes for typing African horsesickness virus isolates using a complete set of cloned VP2-genes08/31/2019
- Diagnosis of African horsesickness08/30/2019
- Diagnostic methods for African horsesickness virus using monoclonal antibodies to structural and non-structural proteins08/29/2019
- Research paperComparative sequence analysis and expression of the M6 gene, encoding the outer capsid protein VP5, of African horsesickness virus serotype nine08/28/2019
- An indirect sandwich ELISA utilising F(ab′)2 fragments for the detection of African horsesickness virus08/27/2019
- Expression and antigenic characterization of the major core protein VP7 of Chuzan virus, a member of the Palyam serogroup orbiviruses08/26/2019
- Development of competitive ELISA for serodiagnosis on African horsesickness virus using baculovirus expressed VP7 and monoclonal antibody08/24/2019
- Baculovirus expression of non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 and their use as antigens in an indirect ELISA08/23/2019
