Research paperComparative sequence analysis and expression of the M6 gene, encoding the outer capsid protein VP5, of African horsesickness virus serotype nine

Add time:08/28/2019    Source:sciencedirect.com

The entire nucleotide and deduced amino acid sequence of the M6 gene of African horsesickness virus (AHSV) serotype nine has been determined from four overlapping cDNA clones. The gene was found to be 1566 bp long, encoding a protein of 505 amino acids with a molecular weight of 56737 Da and a nett charge of −1 at neutral pH. Comparative sequence analysis of the deduced amino acid sequence with the VP5 protein of AHSV-4, showed that only 81% of amino acids were conserved in type and position, although alternating regions of lower and higher conservation was identified by alignment of the primary sequences of different orbiviral VP5 proteins. Antigenically authentic AHSV-9 VP5 was also expressed in a baculovirus expression system and the expressed protein was shown to react specifically with anti-AHSV-9 as well as AHSV-3 serum in Western blot analysis.

We also recommend Trading Suppliers and Manufacturers of VP7 major core antigen, African horsesickness virus (cas 138413-77-1). Pls Click Website Link as below: cas 138413-77-1 suppliers

Prev:An indirect sandwich ELISA utilising F(ab′)2 fragments for the detection of African horsesickness virus
Next:Diagnostic methods for African horsesickness virus using monoclonal antibodies to structural and non-structural proteins)