Induction of salivary kallikreins by the diet containing a sweet-suppressive peptide, gurmarin (cas 138464-10-5), in the rat

Add time:08/24/2019    Source:sciencedirect.com

Gymnema sylvestre (gymnema) contains gurmarin that selectively inhibits responses to sweet substances in rodents. The present study investigated possible interaction between gurmarin and the submandibular saliva in rats fed diet containing gymnema. Electrophoretic analyses demonstrated that relative amounts of two proteins in the saliva clearly increased in rats fed the gymnema diet. However, rats previously given section of the bilateral glossopharyngeal nerve showed no such salivary protein induction. Analyses of amino acid sequence indicate that two proteins are rat kallikrein 2 (rK2) and rat kallikrein 9 (rK9). rK2 and rK9, a family of serine proteases, have a striking resemblance of cleavage site in the protein substrates. Interestingly, gurmarin possesses comparable residues with those rK2 and rK9 prefer. The kallikreins significantly inhibited immunoreaction between gurmarin and antigurmarin antiserum. These results suggest that rK2 and rK9 increased by chemosensory information for the gymnema diet via the glossopharyngeal nerve might cleave gurmarin or at least cause specific binding with it.

We also recommend Trading Suppliers and Manufacturers of gurmarin (cas 138464-10-5). Pls Click Website Link as below: cas 138464-10-5 suppliers

Prev:Handling a tricycle: Orthogonal versus random oxidation of the tricyclic inhibitor cystine knotted peptide gurmarin (cas 138464-10-5)
Next:Electrophysiological characterization of the inhibitory effect of a novel peptide gurmarin (cas 138464-10-5) on the sweet taste response in rats)