Separation and determination of inorganic germanium and β-Carboxyethylgermanium sesquioxide (cas 12758-40-6) by high-performance ion-exclusion chromatography

Add time:08/24/2019    Source:sciencedirect.com

A novel method for the separation and determination of inorganic germanium and β-carboxyethylgermanium sesquioxide (Ge-132) by using high-performance ion-exclusion chromatography was proposed. The separation was performed by an isocratic elution and the determination by conductivity detection in combination with visible absorbance detection at 505 nm in series. Several possible detection modes were compared and the conditions of post-column reaction were optimized. Under the specified experimental conditions, the peak area response was proportional to the concentration of inorganic germanium in the range of 0.05–5 μg ml−1 and of Ge-132 in the range of 0.1–100 μg ml−1, respectively. The method has been successfully applied to the analysis of tonic oral liquids, and the results obtained were in agreement with those of hydride generation atomic fluorescence spectrometry. Additionally, this method has been used for the preliminary study of Ge-132 transformation in vivo: inorganic germanium was not detected in the urine excreted by rats administered Ge-132.

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