Cholesterol recognition system by molecular imprinting on self-assembled monolayer

Add time:08/24/2019    Source:sciencedirect.com

In this study, the molecular imprinting technique and self-assembled monolayer technique were combined to increase the recognition ability of cholesterol. A self-assembled monolayer with 4-mercaptophenol and benzenethiol was formed on a gold plate, and the reaction of cholesteryl chloroformate with the phenol group in the 4-mercaptophenol was followed. This layer was then coated with poly(methyl methacrylate) (PMMA). In order to expose the cholesterol buried under the coating, the partial upper part of the coated PMMA was then removed by stroking the coated plate in acetone solvent. The molecular imprinted site was prepared by hydrolysis of the carbonate bond and extraction of the hydrolyzed cholesterol. This gold plate was used as a working electrode to test the recognition ability for cholesterol. The result showed that the plate obtained good recognition ability for cholesterol compared with cholic acid. The ratio of 4-mercaptophenol to benzenethiol was a very important factor in the ability to recognize cholesterol. Regulating the thickness of the coated PMMA was also one of the important factors to increase the ability to recognize cholesterol.

We also recommend Trading Suppliers and Manufacturers of CHOLESTEROL METHYL CARBONATE (cas 15507-52-5). Pls Click Website Link as below: cas 15507-52-5 suppliers

Prev:EPR study of γ-irradiated cholesteryl methyl carbonate
Next:Effects of DPPC/Cholesterol liposomes on the properties of freshly precipitated calcium carbonate)