Cloning of Xanthomonas DNA that expresses d-xylose catabolic enzymes
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Add time:08/26/2019 Source:sciencedirect.com
A gene bank of the d-xylose utilizing, cellulolytic Xanthomonas (XA1-1) DNA, inserted into the Hind III site of pKT230, was screened for clones which encoded d-xylose isomerase. One clone (pND70) was identified which complemented d-xylose isomerase negative mutants of Escherichia coli and this clone contained an insert of XA1-1 DNA of approximately 15 kb. Enzyme assays showed that pND70 appeared to encode d-xylose permease, and xylulose kinase in addition to d-xylose isomerase. Specific activities of all 3 enzymes from E. coli JA200 (pND70) grown in d-xylose were double those detected in XA1-1 when also grown on d-xylose.
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