OriginalScreening of yeasts for production of xylitol from d-xylose

Add time:08/27/2019    Source:sciencedirect.com

Candida mogii ATCC 18364 was selected for a xylitol producer (YPS=0.62 g/g) from 11 strains of d-xylose utilizing yeasts. Systematic kinetic studies are presented for growth and xylitol formation in synthetic medium using d-xylose as the carbon and energy source. Xylitol is produced from d-xylose under aerobic as well as oxygen-limiting conditions, but not in the absence of oxygen. It can be seen from the exprimental results that the concentrations of d-xylose and dissolved oxygen have a strong influence on the yield and rate for product formation. A maximum product yield was obtained when initial d-xylose concentration and specific oxygen uptake rate were 53 g/l and 0.5 mmol O2/g/h, respectively. Kinetic studies of d-xylose uptake, d-xylose reductase and xylitol dehydrogenase were performed to explain the complex regulatory properties of C. mogii during aerobic and oxygen-limited xylitol formation.

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