Research ArticlePharmaceutical BiotechnologySimple and Robust N-Glycan Analysis Based on Improved 2-Aminobenzoic acid (cas 118-92-3) Labeling for Recombinant Therapeutic Glycoproteins

Add time:08/27/2019    Source:sciencedirect.com

N-glycans of therapeutic glycoproteins are critical quality attributes that should be monitored throughout all stages of biopharmaceutical development. To reduce both the time for sample preparation and the variations in analytical results, we have developed an N-glycan analysis method that includes improved 2-Aminobenzoic acid (cas 118-92-3) (2-AA) labeling to easily remove deglycosylated proteins. Using this analytical method, 15 major 2-AA–labeled N-glycans of Enbrel® were separated into single peaks in hydrophilic interaction chromatography mode and therefore could be quantitated. 2-AA–labeled N-glycans were also highly compatible with in-line quadrupole time-of-flight mass spectrometry (MS) for structural identification. The structures of 15 major and 18 minor N-glycans were identified from their mass values determined by quadrupole time-of-flight MS. Furthermore, the structures of 14 major N-glycans were confirmed by interpreting the MS/MS data of each N-glycan. This analytical method was also successfully applied to neutral N-glycans of Humira® and highly sialylated N-glycans of NESP®. Furthermore, the analysis data of Enbrel® that were accumulated for 2.5 years demonstrated the high-level consistency of this analytical method. Taken together, the results show that a wide repertoire of N-glycans of therapeutic glycoproteins can be analyzed with high efficiency and consistency using the improved 2-AA labeling–based N-glycan analysis method.

We also recommend Trading Suppliers and Manufacturers of 2-Aminobenzoic acid (cas 118-92-3). Pls Click Website Link as below: cas 118-92-3 suppliers

Prev:Direct oxidative cascade cyclisation of 2-Aminobenzoic acid (cas 118-92-3) and arylaldehydes to aryl 4H-3,1-benzoxazin-4-ones with oxone
Next:Microwave-assisted reaction between 2-Aminobenzoic acid (cas 118-92-3)s, 2-hydroxybenzaldehydes, and arylboronic acids: a one-pot three-component synthesis of bridgehead bicyclo[4.4.0]boron heterocycles)