Unique O-ribosylation in the biosynthesis of Butirosin (cas 12772-35-9)

Add time:08/27/2019    Source:sciencedirect.com

Using a comparative genetics approach, one or more of the BtrA, BtrL, BtrP, and BtrV proteins encoded in the butirosin biosynthetic gene cluster (btr) from Bacillus circulans SANK72073 were identified as being responsible for an O-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. Functional analysis of the recombinantly expressed proteins revealed that both BtrL and BtrP were responsible for the ribosylation of neamine, using 5-phosphoribosyl-1-diphosphate (PRPP) as the ribosyl donor. Further detailed analysis indicated that this process occurs via two discrete steps: with BtrL first catalyzing the phosphoribosylaion of neamine to form 5″-phosphoribostamycin, followed by a BtrP-catalyzed dephosphorylation to generate ribostamycin. To the best of our knowledge, this is the first time that the functional characterization of a glycosyltransferase from an aminoglycoside biosynthetic gene cluster has been reported.

We also recommend Trading Suppliers and Manufacturers of Butirosin (cas 12772-35-9). Pls Click Website Link as below: cas 12772-35-9 suppliers

Prev:Chapter 20 Biosynthetic Enzymes for the Aminoglycosides Butirosin (cas 12772-35-9) and Neomycin
Next:ArticleBiosynthesis of Butirosin (cas 12772-35-9): Transfer and Deprotection of the Unique Amino Acid Side Chain)