Biosynthesis of Butirosin (cas 12772-35-9) in Bacillus circulans NRRL B3312: identification by sequence analysis and insertional mutagenesis of the butB gene involved in antibiotic production

Add time:09/03/2019    Source:sciencedirect.com

As an approach to an analysis of the biosynthesis of the aminoglycoside antibiotic Butirosin (cas 12772-35-9) (But), we investigated the chromosomal regions flanking the ButR gene (aphA4/butA) of Bacillus circulans NRRL-B3312, and have identified, by nucleotide sequence analysis, a large open reading frame (ORF; ButB) upstream from the ButR gene. Hybridization was detected between butB and chromosomal DNA from other Bacillaceae that produce But-like compounds (but not from non-producers). Interruption of this sequence by insertion of an erythromycin-resistance-encoding gene (erm) at either of two distinct sites eliminated the production (biosynthesis or export) of But, thus indicating a role for butB in antibiotic production. Gene butB is transcribed in the same direction as but A and encodes a protein of 1616 amino acid (aa) residues with a 30-aa N-terminal signal peptide. Comparison of the sequence for the translation product (ButB) with the aa compositions and sequences of known bacterial surface proteins, such as S-layer proteins, suggests that this protein is cell-wall associated. It is proposed that ButB plays a role in the export of But from the producing organism.

We also recommend Trading Suppliers and Manufacturers of Butirosin (cas 12772-35-9). Pls Click Website Link as below: cas 12772-35-9 suppliers

Prev:Carbon-13 n.m.r. Spectra of the antibiotic Butirosin (cas 12772-35-9) A, and related aminoglycosides
Next:An approach to on-line electrospray mass spectrometric detection of polypeptide antibiotics of Enramycin (cas 12772-37-1) for high-speed counter-current chromatographic separation)