Kinetic resolution of (R,S)-ethyl 2-hydroxyl-4-phenylbutyrate via lipase-catalyzed hydrolysis and transesterification in isooctane

Add time:09/05/2019    Source:sciencedirect.com

In the enantioselective hydrolysis of (R,S)-ethyl 2-hydroxy-4-phenylbutyrate ((R,S)-EHPB) in isooctane or in a biphasic solution, Lipase PS having an enantiomeric ratio (i.e. the E value) of 22 for the (S)-enantiomer was screened as the best lipase to produce the remaining (R)-ethyl 2-hydroxy-4-phenylbutyrate ((R)-EHPB). When (R)-2-hydroxy-4-phenylbutanoic acid ((R)-HPBA) was the desired product, one might employ Novozym 435, possessing (R)-stereoselectivity with E=22, as the biocatalyst. The results of using Lipase PS in the enantioselective transesterification of (R,S)-EHPB in isooctane indicated that addition of more vinyl acetate (VA) could suppress the hydrolysis side-reaction, leading to an apparent E value >100. The result of E>100 was also found when initial VA concentration was fixed at 80 mM and (R,S)-EHPB concentrations increased from 5.4 to 190 mM. Vinyl laurate was further selected as the best acyl donor after comparing the enzyme performances among all vinyl esters employed and considering the easiness of product separation in the down-stream process.

We also recommend Trading Suppliers and Manufacturers of ethyl 2-phenylbutyrate (cas 119-43-7). Pls Click Website Link as below: cas 119-43-7 suppliers

Prev:Microbial reduction of ethyl 2-oxo-4-phenylbutyrate. Searching for R-enantioselectivity. New access to the enalapril like ACE inhibitors
Next:Research paperMembrane reactor development for the kinetic resolution of ethyl 2-hydroxy-4-phenylbutyrate)