Inhibition of integrin signaling with Arg-Gly-Asp motifs in rat hepatic stellate cells

Add time:09/02/2019    Source:sciencedirect.com

Background/Aims: Activation of hepatic stellate cells plays a key role in liver fibrogenesis. Disruption of normal hepatic stellate cell-matrix interactions may contribute to this process. However, little is known about the molecular events leading from integrin-extracellular matrix interaction to hepatic stellate cell function. Therefore, we investigated the role of integrin signaling in tyrosine phosphorylation of focal adhesion kinase and cytoskeletal assembly in rat hepatic stellate cells using soluble Arg-Gly-Asp containing peptides.Methods: Hepatic stellate cells were isolated from normal rat livers. Integrin α5β1 expression in hepatic stellate cells was analyzed by immunoprecipitation and immunocytochemistry. The cytoskeletal assembly and tyrosine phosphorylation of focal adhesion kinase were determined by immunocytochemistry and immunoblotting. We also analyzed the effect of Arg-Gly-Asp containing peptides on the expression of smooth muscle α actin by immunocytochemistry and immunoblotting.Results: We identified integrin α5β1 in rat hepatic stellate cells. Stress fiber formation and cell shape were different when hepatic stellate cells were plated on various extracellular matrix components. Treatment of hepatic stellate cells with soluble Arg-Gly-Asp peptides diminished the adhesion-induced tyrosine phosphorylation focal adhesion kinase and inhibited the formation of stress fibers. The peptides also reduced the expression of smooth muscle α actin.Conclusions: Our results demonstrate that adhesion to extracellular matrix induces tyrosine phosphorylation of focal adhesion kinase and promotes actin stress fiber formation and focal adhesion assembly in rat hepatic stellate cells, and that these events are disturbed by soluble Arg-Gly-Asp peptides.

We also recommend Trading Suppliers and Manufacturers of aprotinin, Arg(15)-Glu(52)- (cas 114264-91-4). Pls Click Website Link as below: cas 114264-91-4 suppliers

Prev:Studies on curare-like action of the tripeptide carbobenzoxy-Gly-Gly-Arg-β-naphthylamide in mouse diaphragm
Next:BJ-48, a novel thrombin-like enzyme from the Bothrops jararacussu venom with high selectivity for Arg over Lys in P1: Role of N-glycosylation in thermostability and active site accessibility)