ArticleSplit-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy
-
Add time:08/30/2019 Source:sciencedirect.com
SummaryA protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as “multicolor” electron microscopy (EM) via the photooxidation of 3-3′-diaminobenzidine and its derivatives.
We also recommend Trading Suppliers and Manufacturers of M-twist protein (cas 136253-27-5). Pls Click Website Link as below: cas 136253-27-5 suppliers
Prev:Mouse strain differences in immobility and sensitivity to fluvoxamine and desipramine in the forced swimming test: Analysis of serotonin and noradrenaline transporter (cas 136253-20-8) binding
Next:Differences between ultrastructure and protein composition in straight hair fibres) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- The aqueous environment as an active participant in the protein folding process09/08/2019
- ArticleStructure of the MORN4/Myo3a Tail Complex Reveals MORN Repeats as Protein Binding Modules09/07/2019
- Thiol-activated fluorescent probe for sensitive detection and imaging of proteins09/06/2019
- How B-DNA Dynamics Decipher Sequence-Selective Protein Recognition09/05/2019
- Fate of the fluorescent state of p-amido analogue of green fluorescence protein chromophore09/04/2019
- Diffracted X-ray tracking method for recording single-molecule protein motions09/03/2019
- Historical perspectiveFood protein amyloid fibrils: Origin, structure, formation, characterization, applications and health implications09/02/2019
- Full paperThe M-twist gene of Mus is expressed in subsets of mesodermal cells and is closely related to the Xenopus X-twi and the Drosophila twist genes☆09/01/2019
- Differences between ultrastructure and protein composition in straight hair fibres08/31/2019
-
Health and Chemical more >