In vitro DNA binding of 2-amino-3-methylimidazo(4,5-ƒ)[5-3H]quinoline (IQ) is modulated by sulfotransferase and sulfate levels

Add time:09/02/2019    Source:sciencedirect.com

DNA binding in vitro was measured with 2-amino-3-methylimidazo(4,5-ƒ)[5-3H]quinoline (3H-IQ) in the presence of S9 and microsomes from Wistar rat liver. DNA binding of 3H-IQ was catalyzed by both microsomes and S9 and was increased 5-fold in Aroclor (PCB)-pretreated animals. DNA binding was reduced by the specific inhibitor of sulfotransferase 2,6-dichloro-4-nitrophenol and slightly increased by 3′-phosphoadenosine 5′-phosphosulfate. The incubation of IQ in media with increasing concentrations of sulfate produced a modest dose-dependent increase in DNA binding.Liver S9 obtained from rats which had been administered 1% sulfite in drinking water catalyzed a higher binding of IQ to DNA, and this effect was inhibited by pentachlorophenol. The data demonstrate that sulfotransferase has a role in the activation of IQ and that variations in the activity of this enzyme induced by dietary changes may vary the genotoxic activity of IQ.

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