Macrolactonization to 10-deoxymethynolide catalyzed by the recombinant thioesterase of the picromycin (cas 19721-56-3)/methymycin polyketide synthase

Add time:09/01/2019    Source:sciencedirect.com

The recombinant thioesterase (TE) domain of the picromycin/methymycin synthase (PICS) catalyzes the macrolactonization of 3, the N-acetylcysteamine thioester of seco-10-deoxymethynolide to generate 10-deoxymethynolide (1) with high efficiency. By contrast, 4, the 7-dihydro derivative of seco-thioester 3, undergoes exclusive hydrolysis by PICS TE to seco-acid 5. The recombinant TE domain of 6-deoxyerythronolide B synthase (DEBS TE) shows the same reaction specificity as PICS TE, but with significantly lower activity.

We also recommend Trading Suppliers and Manufacturers of picromycin (cas 19721-56-3). Pls Click Website Link as below: cas 19721-56-3 suppliers

Prev:Elucidating the mechanism of chain termination switching in the picromycin (cas 19721-56-3)/methymycin polyketide synthase
Next:Macrolide biosynthesis: A single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin (cas 19721-56-3) in Streptomyces venezuelae)