The determination of thiols and of total glutathione in human blood using 6,6′-dithiodinicotinic acid (CPDS)

Add time:09/03/2019    Source:sciencedirect.com

Two spectrophotometric methods are described for the rapid and accurate determination of thiols and of total glutathione in human blood. Both methods rely on the conversion of 6,6′-dithiodinicotinic acid by thiols to 6-mercaptonicotinic acid. The methods permit: (a) the specific determination of thiols at levels of 1 μg SH (equivalent to 10 μg glutathione), and (b) the specific determination of total glutathione at levels of 0.3 μg. The latter method is based on a specific enzymatic cycling reaction of glutathione. Both determinations may be performed rapidly and easily using a standard spectrophotometer.

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