Spermine-NBD as fluorescent probe for studies of the polyamine transport system in Leishmania donovani
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Add time:09/09/2019 Source:sciencedirect.com
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a potent IC50 of 60 µM.
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