Short communicationSeparation of hexabromocyclododecane diastereomers: Application of C18 and phenyl-hexyl ultra-performance liquid chromatography columns

Add time:09/02/2019    Source:sciencedirect.com

This study was performed to establish the proper liquid chromatographic conditions for the separation of hexabromocyclododecane (HBCD) diastereomers. Column selectivity towards HBCD diastereomers was evaluated for C18 and phenyl-hexyl stationary phases. First, the baseline separation of the primary HBCDs (α-, β-, and γ-HBCD) was obtained using the ultra-performance liquid chromatography (UPLC) column with C18 stationary phase chosen in most previous studies for HBCD analysis; however, co-elution of δ- and ε-HBCD with the primary HBCD diastereomers was observed. To prevent the interference from δ- and ε-HBCD, we adopted a phenyl-hexyl UPLC column to resolve the HBCD diastereomers. The phenyl-hexyl UPLC column showed significantly different selectivity for the HBCD diastereomers compared with the C18 column, which allowed the clear isolation of δ-HBCD and ε-HBCD from the primary HBCD diastereomers. In addition, by checking the retention times of all HBCD diastereomers using both C18 and phenyl-hexyl columns, we confirmed the presence of δ-, ε-, η-, and θ-HBCDs in two technical HBCD mixtures.

We also recommend Trading Suppliers and Manufacturers of hexyl (Z)-docos-13-enoate (cas 19773-56-9). Pls Click Website Link as below: cas 19773-56-9 suppliers

Prev:Kinetics of the reactions of OH radicals with n-butyl, isobutyl, n-pentyl and 3-methyl-1-butyl nitrates
Next:The effect of 1-hexyl-3-methylimidazolium bromide ionic liquid as a co-solvent on the aqueous solubility of lamotrigine at T = (293.2–313.2) K)