Regular ArticleCCAAT/Enhancer-Binding Protein δ Gene Expression Is Mediated by Autoregulation through Downstream Binding Sites☆

Add time:09/04/2019    Source:sciencedirect.com

CCAAT/enhancer-binding protein δ (C/EBPδ) transcription factor is sharply induced at the early stage of the acute phase response. We previously reported that the C/EBPδ gene expression is induced by the acute-phase response factor/signal transducers and activators of transcription 3 (APRF/STAT3). However, the expression level of the C/EBPδ gene is relatively high up to several hours after the stimulation, whereas APRF/STAT3 is inactivated within one hour. In this report, we identified the two C/EBPδ binding sites at the downstream region of this gene. The binding analysis revealed that both of these sites bound recombinant C/EBPδ protein. A cotransfection analysis identified these sites as thecis-elements for the autoregulation. We conclude that the C/EBPδ gene is activated by APRF/STAT3, and the expression level is then maintained by an autoregulation mechanism.

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