Synthesis, pH-induced “on–off–on” luminescence switching, and partially intercalative DNA-binding and DNA photocleavage properties of an β-d-allopyranoside-grafted ruthenium(II) complex

Add time:09/04/2019    Source:sciencedirect.com

A new ruthenium(II) complex [Ru(Happip)3](ClO4)2 {Happip = 2-(4-(β-d-allopyranoside)phenyl)imidazo[4,5-f][1,10]phenanthroline} was synthesized and characterized by elemental analysis, 1H NMR and matrix-assisted laser desorption ionization mass spectrometry. Calf-thymus DNA-binding properties were studied by DNA viscosity measurements and spectroscopic methods of UV-visible (UV-vis) absorption and luminescence titrations, steady-state emission quenching, DNA competitive binding with ethidium bromide and DNA melting experiments, indicating that the complex partially intercalates into the DNA with a large binding constant greater than 106 M− 1. The pH effects on UV-vis absorption and emission spectra of the complex were studied, demonstrating that the complex acted as an excellent pH-induced “on–off–on” luminescence switch with large on–off intensity ratios of 88 and 50 with one of luminescence on/off switching actions occurring in near-physiological pH region (pKa2 = 7.33). The DNA photocleaving properties of [Ru(Happip)3]2+ were also studied and compared with those of [Ru(bpy)2(Happip)]2+ and [Ru(bpy)2(Hpip)]2+{bpy = 2,2′-bipyridine, Hpip = 2-(4-phenyl)imidazo[4,5-f][1,10]phenanthroline}.

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