The fluorescence studies of interaction between 4-(n-2′-glucosyl) butyramidyl triphenyl phosphonium chloride and DNA

Add time:09/03/2019    Source:sciencedirect.com

A new compound 4-(n-2′-glucosyl) butyramidyl triphenyl phosphonium chloride (4-GBTPC) was synthesized, and its interactions with nucleic acid were studied by fluorescence method. The results of the experiment indicated that DNA can obviously decrease the fluorescence intensity of 4-GBTPC by the static quenching style. DNA could be determined by using of the fluorescence quenching action of DNA on 4-GBTPC. The linear range was 8.9×10−6 to 1.16×10−4 mol L−1 with the detection limit of 9.1×10−7 mol L−1. Effects of interference substances and optimization of procedure were investigated. Three synthetic samples were determined with satisfaction.

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