Convenient chemoenzymatic synthesis of β-purine-diphosphate sugars (GDP-fucose-analogues)
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Add time:09/10/2019 Source:sciencedirect.com
A series of peracetylated β-sugar-1-phosphates with l-fuco configuration are efficiently prepared chemically and coupled in high yield to purine monophosphate bases via imidazolide activation. The resulting purine diphosphate sugars are deacetylated completely by a mild treatment with commercial acetylesterase (EC 3.1.1.6) to give donor-substrates for fucosyltransferases.
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