Short crystallization paperCrystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A2 complexed with p-bromophenacyl bromide and α-tocopherol inhibitors at 1.9- and 1.45-Å resolution

Add time:09/09/2019    Source:sciencedirect.com

An acidic phospholipase A2 (PLA2) isolated from Bothrops jararacussu snake venom was crystallized with two inhibitors: α-tocopherol (vitamin E) and p-bromophenacyl bromide (BPB). The crystals diffracted at 1.45- and 1.85-Å resolution, respectively, for the complexes with α-tocopherol and p-bromophenacyl bromide. The crystals are not isomorphous with those of the native protein, suggesting the inhibitors binding was successful and changes in the quaternary structure may have occurred.

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