A convenient synthesis of amino acid p-nitroanilides; synthons in the synthesis of protease substrates

Add time:09/27/2019    Source:sciencedirect.com

A method is described for the synthesis of Nα-protected bi- and trifunctional amino acid p-nitroanilides. The reaction uses phosphorus oxychloride as the condensing agent. The synthesis is simple, rapid, free of racemization and affords yields between 70–90%. The synthesis can be performed not only with amino acid derivatives of the urethane type including acid-labile (Z. Boc) and base-labile (Fmoc, Msc) Nα-proteclivc functions or allyl-derived protections, but also with Nα-trilyl amino acids, albeit in lower yield. The reaction runs in pyridine and its mechanism implies carboxyl activation by formation of a mixed anhydride with phosphorodichloridic acid (HOPOCl2).

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