CommunicationsPLP catabolism: Identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099

Add time:09/07/2019    Source:sciencedirect.com

The function of the mlr6793 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 4-pyridoxic acid dehydrogenase, an enzyme involved in the catabolism of PLP (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. 4-Pyridoxic acid dehydrogenase is a 33 kDa protein that catalyzes the four electron oxidation of 4-pyridoxic acid to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate, using nicotinamide adenine dinucleotide as a cofactor. The kcat for NADH production is 0.01 s−1. The KM values for 4-pyridoxic acid and NAD are 5.8 and 6.6 μM, respectively.

We also recommend Trading Suppliers and Manufacturers of 4-Hydroxy-2-methylpyridine (cas 18615-86-6). Pls Click Website Link as below: cas 18615-86-6 suppliers

Prev:Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase from Mesorhizobium loti
Next:Ipso-nitration of carboxylic acids using a mixture of nitronium tetrafluoroborate, base and 1-hexyl-3,4,5-trimethyl-1H-imidazolium tetrafluoroborate)