High-performance liquid chromatographic separation of phenols on a fluorocarbon-bonded silica gel column

Add time:09/24/2019    Source:sciencedirect.com

A branched-chain, fluorocarbonaceous, silane-bonded silica gel material for high-performance liquid chromatography (HPLC) has been developed. New columns were found to have a high ability to recognize halogen-containing molecules and separate geometrical isomers of halogen-containing phenols. The standard mixture for the EPA 604 method of analysis of many halogenous phenols was separated better than with gas chromatography. These fluorocarbon-bonded columns also separated various types of phenols without a halogen atom. Polyphenols such as flavonoids are separated with a sharp peak shape and excellent durability under extreme eluting conditions, in contrast with ordinary hydrocarbon-bonded silica gel columns.

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