Simple and sensitive method for the quantification of total bilirubin in human serum using 3-Methyl-2-benzothiazolinone (cas 18644-20-7) hydrazone hydrochloride as a chromogenic probe

Add time:09/25/2019    Source:sciencedirect.com

We here describe a new spectrophotometric method for measuring total bilirubin in serum. The method is based on the cleavage of bilirubin giving formaldehyde which further reacts with diazotized 3-Methyl-2-benzothiazolinone (cas 18644-20-7) hydrazone hydrochloride giving blue colored solution with maximum absorbance at 630 nm. Sensitivity of the developed method was compared with Jendrassik-Grof assay procedure and its applicability has been tested with human serum samples. Good correlation was attained between both methods giving slope of 0.994, intercept 0.015, and R2 = 0.997. Beers law obeyed in the range of 0.068–17.2 μM with good linearity, absorbance y = 0.044Cbil + 0.003. Relative standard deviation was 0.006872, within day precision ranged 0.3–1.2% and day-to-day precision ranged 1–6%. Recovery of the method varied from 97 to 102%. The proposed method has higher sensitivity with less interference. The obtained product was extracted and was spectrally characterized for structural confirmation with FT-IR, 1H NMR.

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