Original PapersHorseradish peroxidase-catalyzed oxidative coupling of 3-methyl 2-benzothiazolinone hydrazone and methoxyphenols

Add time:09/26/2019    Source:sciencedirect.com

The reaction between o-, m-, and p-methoxyphenols and 3-methyl-2-benzothiazolinone hydrazone (MBTH) is studied in the presence of horseradish peroxidase (HRP) and H2O2 as oxidative agent. The findings indicate that enzyme (H2O2 oxidoreductase; EC 1.11.1.7) catalyzes an oxidative coupling reaction between MBTH and phenols which produces azo dye compounds. On the basis of kinetic parameters and optimum pH values, a mechanism in which both MBTH and phenols seem to be activated by the HRP for achieving the oxidative coupling is proposed. Furthermore, in the current study, we have evaluated the possibility that these azo dyes may be useful in the measurement of peroxidase activity. The method is based on the observed increase in the absorbance at 502 nm (8,355 cm−1 m−1 of extinction molar coefficient) due to the formation of a red azo dye compound resulting from the peroxidase-catalyzed oxidative coupling of MBTH and o-methoxyphenol (guaiacol). Using this assay system, HRP can be determined in picomolar levels by a fixed time method.

We also recommend Trading Suppliers and Manufacturers of 3-Methyl-2-benzothiazolinone (cas 18644-20-7). Pls Click Website Link as below: cas 18644-20-7 suppliers

Prev:Simple and sensitive method for the quantification of total bilirubin in human serum using 3-Methyl-2-benzothiazolinone (cas 18644-20-7) hydrazone hydrochloride as a chromogenic probe
Next:Kinetic method for the determination of iron(II, III) by its catalytic effect on the oxidation of 3-Methyl-2-benzothiazolinone (cas 18644-20-7) hydrazone with hydrogen peroxide)