Original research articleProduction of 2-methyl-1-butanol and 3-Methyl-1-butanol (cas 123-51-3) in engineered Corynebacterium glutamicum
-
Add time:09/08/2019 Source:sciencedirect.com
The pentanol isomers 2-methyl-1-butanol and 3-Methyl-1-butanol (cas 123-51-3) represent commercially interesting alcohols due to their potential application as biofuels. For a sustainable microbial production of these compounds, Corynebacterium glutamicum was engineered for producing 2-methyl-1-butanol and 3-methyl-1-butanol via the Ehrlich pathway from 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. In addition to an already available 2-ketoisocaproate producer, a 2-keto-3-methylvalerate accumulating C. glutamicum strain was also constructed. For this purpose, we reduced the activity of the branched-chain amino acid transaminase in an available C. glutamicum l-isoleucine producer (K2P55) via a start codon exchange in the ilvE gene enabling accumulation of up to 3.67 g/l 2-keto-3-methylvalerate. Subsequently, nine strains expressing different gene combinations for three 2-keto acid decarboxylases and three alcohol dehydrogenases were constructed and characterized. The best strains accumulated 0.37 g/l 2-methyl-1-butanol and 2.76 g/l 3-methyl-1-butanol in defined medium within 48 h under oxygen deprivation conditions, making these strains ideal candidates for additional strain and process optimization.
We also recommend Trading Suppliers and Manufacturers of 3-Methyl-1-butanol (cas 123-51-3). Pls Click Website Link as below: cas 123-51-3 suppliers
Prev:A method for determination of aldosterone in adrenal tributary venous serum by derivatization using Girard P reagent isotopologues followed by LC/ESI-MS/MS
Next:Liquid–liquid equilibrium for the ternary system of 1-butanol + 3-Methyl-1-butanol (cas 123-51-3) + water at different temperatures) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Original articleDensities, viscosities, and refractive indices for binary and ternary mixtures of formamide (1) + N,N-dimethylacetamide (2) + 2-methyl-1-butanol (3) at 298.15 K for the liquid region and at ambient pressure09/26/2019
- Ternary-phase equilibria for CO2 + 3-Methyl-1-butanol (cas 123-51-3) + 2-phenylethanol09/25/2019
- Kinetics of growth and 3-Methyl-1-butanol (cas 123-51-3) production by meat-borne, coagulase-negative staphylococci in view of sausage fermentation09/24/2019
- Isothermal vapor-liquid equilibria, excess molar volume and the deviation of refractive indices for binary mixtures of 1-butanol, 1-hexanol, 3-Methyl-1-butanol (cas 123-51-3) and butyl acetate09/10/2019
- Liquid–liquid equilibrium for the ternary system of 1-butanol + 3-Methyl-1-butanol (cas 123-51-3) + water at different temperatures09/09/2019