Ratiometric fluorescent detection of Azodicarbonamide (cas 123-77-3) based on silicon nanoparticles and quantum dots

Add time:09/09/2019    Source:sciencedirect.com

Herein, a novel ratiometric fluorescent probe ([email protected]) was constructed for quantitative detection of ADA in flour for the first time. The ratiometric fluorescent probe ([email protected]) based on silicon nanoparticles (SiNPs) and CdTe quantum dots (QDs) showed two well-resolved emission peaks at 445 nm and 632 nm, respectively. The fluorescence peak at 632 nm was quenched by mercury ions (Hg2+) through ion-binding and electron transfer processes. However, with the presence of glutathione (GSH), the fluorescence peak at 632 nm was recovered due to the strong affinity between the sulfydryl of GSH and Hg2+. Further, after adding ADA, the sulfydryl (SH) was oxidized into disulfide bond (SS), and Hg2+ was released from the sulfydryl of GSH and quenched the fluorescence at 632 nm again, whereas the fluorescence intensity of [email protected] at 445 nm was unchanged all the time. It exhibited an excellent linear relationship between the fluorescence intensity ratio (F632/F445) and the concentration of ADA in the range of 0.05–4 μM with a detection limit of 0.016 μM. Furthermore, the established method was successfully utilized to the detection of ADA in flour with satisfactory results.

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