Research ArticlesGLC Determination of ETHINAMATE (cas 126-52-3) and Its Hydroxy Derivative in Biological Fluids

Add time:09/09/2019    Source:sciencedirect.com

A sensitive, specific GLC assay was developed for the determination of ETHINAMATE (cas 126-52-3) in plasma and its major metabolite, trans-4-hydroxyethinamate, in urine. The assay uses a mass internal standard of dimethylethinamate. Ethinamate is extracted from alkalinized plasma with dichloromethane. Urine samples require β-glucuronidase hydrolysis prior to extraction of hydroxyethinamate. The dichloromethane is removed by evaporation, and the compounds are measured by GLC using a flame-ionization detector. By using GLC-chemical-ionization mass spectrometry, the compounds measured were identified as the intact ethinamates. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method. From these data, the ethinamate plasma half-life was calculated as 1.9 ± 0.3 hr.

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