Research letterReceptor of Trans-Zeatin (cas 1673-39-4) involved in transcription activation by cytokinin

Add time:09/27/2019    Source:sciencedirect.com

Zeatin-binding protein (67 ± 2 kDa) was isolated from the cytosol of the first leaf of 10-day-old barley plants. The protein fits to all requirements for a zeatin receptor: (i) it binds [3H]Trans-Zeatin (cas 1673-39-4) reversibly and specifically, (ii) it is recognized by anti-idiotype antibodies from antiserum raised against trans-zeatin, (iii) in concert with 10−8 M trans-zeatin it activates rRNA synthesis in vitro in a transcription elongation system containing chromatin from barley leaves associated with RNA-polymerase I. In the presence of trans-zeatin, the protein activates also RNA synthesis directed by RNA-polymerase I and RNA-polymerase II in isolated nuclei from barley leaves.

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