RESEARCH PAPERIndirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Tylosin in Milk and Water Samples

Add time:09/26/2019    Source:sciencedirect.com

A specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were optimized as follows: the coating antigen and antibody were respectively diluted by 6000 times and 2000 times, and the competitive reaction time was 40 min. Under the optimized conditions, the method showed a detection limit of 0.07 ng mL−1, an IC50 of 1.39 ng mL−1 and a linear range of 0.17–11.0 ng mL−1. The recoveries of spiked raw milk and water samples were ranged from 78.4% to 105.6%, with the RSDs of less than 15%. Good correlation between icELISA and HPLC method was obtained for spiked samples (R2 = 0.97). This method was suitable for the determination of tylosin residues in milk and water samples.

We also recommend Trading Suppliers and Manufacturers of Tylosin phosphate (cas 1405-53-4). Pls Click Website Link as below: cas 1405-53-4 suppliers

Prev:Size-selective toxicity effects of the antimicrobial tylosin on estuarine phytoplankton communities☆
Next:Spectroscopic analysis of tylosin adsorption on extracellular DNA reveals its interaction mechanism)