Regular paperEffect of geographic origin, variety and storage on tocopherol concentrations in walnuts by HPLC

Add time:10/01/2019    Source:sciencedirect.com

Retinol, tocopherols and carotenes were extracted from walnut kernels. Three walnut extraction procedures were compared. Direct hexane extraction was selected. The fat-soluble vitamin separation was achieved by reversed-phase high performance liquid chromatography equipped with a ternary mobile phase gradient (methanol, butanol, water), and wavelength programming. The chromatographic run was under 35 minutes. This technique allowed a good resolution of α, γ, δ tocopherols, α and β carotenes and retinol.Two walnut varieties (Franquette and Hartley) from two geographical origins (France, USA) were analyzed. Retinol, and carotenes were not identified in walnuts. The amounts of α, γ, δ tocopherols ranged respectively from 1.08 to 4.05, 21.78 to 26.46, and 2.51 to 4.23 (mg/100 g).Significant differences related to variety and geographical origin were observed. A decrease in tocopherols (about 30%) was observed after 3 month refrigerated storage.

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