A two-step, one-pot enzymatic synthesis of ampicillin from penicillin G potassium salt

Add time:09/30/2019    Source:infona.pl

A two-step, one-pot synthesis of ampicillin from penicillin G potassium salt (PGK) in aqueous buffer/organic co-solvent has been achieved. Ethylene glycol (EG) was chosen as the organic co-solvent. Factors including co-solvent content, enzyme loading, reaction temperature and substrate concentration were investigated. The optimum conditions were as follow: pH 8.0 phosphate buffer solution, 50% EG (v/v), 25°C, 100mM PGK and 300mM d-phenylglycine methyl ester (D-PGM), 43.2IU/ml IPA-750. The maximum yield was 57.3% after a reaction time of 17h. It is the first report about the synthesis of ampicillin from penicillin G potassium salt in one-pot combining the enzymatic hydrolysis and the subsequent enzymatic condensation, and the novel methodology will have important application in the β-lactam antibiotics industry.

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