The enzymatic synthesis of geranyl geranyl pyrophosphate by enzymes of carrot root and pig liver

Add time:07/18/2019    Source:sciencedirect.com

The isolation of geranyl geranyl pyrophosphate synthetases from carrot root and pig liver and the properties of these enzymes are reported. Each of the enzymes catalyzes the formation of geranyl geranyl pyrophosphate from isopentenyl-4-C14 and farnesyl pyrophosphates. The product of the reaction has been characterized by gas-liquid and paper chromatography and derivative formation. An absolute metal requirement has been demonstrated for the reaction and Mn++ is the most effective metal in stimulating activity. The pH optimum of the reaction is 6.7–6.8 and 6.9–7.0, respectively, for the carrot root and pig liver enzymes. Km values calculated for farnesyl pyrophosphate and isopentenyl pyrophosphate are 1 × 10−4M and 1 × 10−5M respectively, for the carrot root enzyme, and 7.6 × 10−5M and 6.6 × 10−6M, respectively, for the pig liver enzyme.

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