Journal of Organic Chemistry p. 70 - 77 (1989)
Update date:2022-09-26
Topics:
Drueckhammer, Dale G.
Durrwachter, J. Robert
Pederson, Richard L.
Crans, Debbie C.
Daniels, Lacy
Wong, Chi-Huey
A synthetic strategy is developed that uses organic phosphate utilizing enzymes as catalysts and a mixture of an organic alcohol and inorganic arsenate or vanadate to replace the organic phosphate substrate.In this process, inorganic arsenate or vanadate reacts with the alcohol reversibly in situ to form a mixture of esters, one of which is accepted by the enzyme as a substrate.Examples of the utility of this approach are demonstrated in enzymatic aldol condensations catalyzed by fructose-1,6-diphosphate aldolase, fuculose-1-phosphate aldolase, and rhamnulose-1-phosphate aldolase with a mixture of dihydroxyacetone and inorganic arsenate as substrate.Several uncommon sugars and deoxy sugars are prepared on 5-17-mmol scales.Mechanistic studies on an aldol reaction indicate that the redox reaction between dihydroxyacetone and inorganic vanadate prohibits the use of such a mixture to replace dihydroxyacetone phosphate in enzymatic aldol condensations.
View MoreContact:+86-29-88710656
Address:South Tai bai Road, High Tech Development Zone, Xi'an China
Anhui Jiatiansen Agrochemical Co.,Ltd
Contact:15366811918
Address:chemical industrial park,xiangyu town,dongzhi county,anhui,china
Global United Biotechnology Inc.
Contact:+86-21-61618568
Address:Room 309, Building 6, NO.135, Jinyu Road, Pudong
Zhangjiagang Jianing Import & Export Co.,Ltd.
Contact:086-512-55379012 13913607595
Address:NO.1 Guotai North Road Zhangjiagang Economic Development Zone,215600,Jiangsu,China
Changzhou Anyi Biochem Co., Ltd.(expird)
Contact:+86-519-88836158
Address:no,51 caoda
Doi:10.1007/BF00961931
(1988)Doi:10.1039/P29880001473
(1988)Doi:10.1039/b708952j
(2007)Doi:10.1248/cpb.36.515
(1988)Doi:10.1246/cl.1988.809
(1988)Doi:10.1002/chem.200901369
(2009)