Enantiopure derivatives of aza-baylis-hillman adducts by subsequent sn'-sn' reactions of acylcarbamates bearing a chiral auxiliary

Article abstract of DOI:10.3390/molecules14082824

Reactions of (4S,5R)-1-(3,4-Dimethyl-2-oxo-5-phenylimidazolidine) carbonylisocyanate (4) with appropriate Baylis-Hillman adducts 5 gave the corresponding acyl carbamates 6,7 as equimolar diastereomeric mixtures. These mixtures were treated with DABCO, to afford with moderate diastereoselection easily separable [2-(3",4"-dimethyl-2"-oxo-5"- phenylimidazolidine-1-carboxamido)(aryl)methyl]acrylates 8 and 9.

Full text of DOI:10.3390/molecules14082824

Molecules 2009, 14, 2824-2835; doi:10.3390/molecules14082824
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Communication
Enantiopure Derivatives of Aza-Baylis-Hillman Adducts by
Subsequent SN′-SN′ Reactions of Acylcarbamates Bearing a
Chiral Auxiliary
Gianluca Martelli, Eleonora Marcucci, Mario Orena * and Samuele Rinaldi
Dipartimento I.S.A.C. - Università Politecnica delle Marche, Via Brecce Bianche - I-60131 Ancona,
Italy; E-mail: srinaldi@univpm.it (S.R.)
* Author to whom correspondence should be addressed; E-mail: m.orena@univpm.it
Received: 7 July 2009; in revised form: 22 July 2009 / Accepted: 30 July 2009 /
Published: 30 July 2009
Abstract: Reactions of (4S,5R)-1-(3,4-Dimethyl-2-oxo-5-phenylimidazolidine)carbonyl-
isocyanate (4) with appropriate Baylis-Hillman adducts 5 gave the corresponding acyl
carbamates 6,7 as equimolar diastereomeric mixtures. These mixtures were treated with
DABCO, to afford with moderate diastereoselection easily separable [2-(3",4"-dimethyl-
2"-oxo-5"-phenylimidazolidine-1-carboxamido)(aryl)methyl]acrylates 8 and 9.
Keywords: chiral auxiliary; aza-Baylis-Hillman; rearrangement
Introduction
Biological processes are mainly governed by the interaction of peptides with macromolecular
receptors. For these interactions to be successful, the three-dimensional conformation of the peptide
chain is of crucial importance, and considerable efforts have been directed towards improving the
pharmacological properties of natural peptides by structure modifications of the amino acid
constituents [1-3]. Thus, in recent years research has largely focused on the incorporation of
stereochemically constrained amino acids into peptides, with the aim of effectively reducing the
populations of possible peptide chain conformations, enhancing in turn potency, receptor selectivity
and pharmacokinetic properties [4-6]. In addition, modifications which decrease conformational
mobility are useful in order to gain insight into the relationship between the biological activity and the
three dimensional topology, new procedures aimed to enforce conformational restrictions in peptides

Molecules 2009, 14
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are welcome. In this field, we sought to prepare enantiomerically pure derivatives of α-methylene β-
amino acids [7-10], which can be also obtained by aza-Baylis-Hillman reactions [11-12]. In fact, these
compounds deserve interest in their own right due to the conformational restriction of the double bond
[13]. In addition, the functionalization of the double bond leads to an α-quaternary centre, also able to
induce conformational restrictions [14].
Results and Discussion
The use of chiral auxiliaries for controlling the stereochemistry of numerous reactions is well
established. Thus, in recent years imidazolidin-2-one 1 and its enantiomer, easily obtained starting
from either enantiomer of ephedrine [15], were employed as versatile chiral auxiliaries in a lot of
synthetically useful reactions [16-18]. In fact, on addition of a solution of the lithium anion of the
compound 1 in THF to a solution of phosgene in toluene at -78 °C, the corresponding chloride 2 was
obtained [18], which was subsequently converted into the derivative 3 by reaction with a 30% solution
of ammonia (Scheme 1) [19]. Following the literature method, compound 3 was converted into the
corresponding acyl isocyanate 4 [20].
Scheme 1. Synthesis of (4S,5R)-1-(3,4-Dimethyl-2-oxo-5-phenylimidazolidine)carbonyl-
isocyanate (4).
O
O
O
NH2
O
Cl
i
ii
iii
H
N
N
N
N
N
N
O
1
2
3
O
C
O
N
N
N
O
4
Reagents and Conditions: i. (a) n-BuLi, THF, 0 °C; (b) phosgene, -78 °C; ii. 30% NH4OH,
overall yield 90%; iii. oxalyl chloride, refluxing DCE.
The solution containing compound 4 was immediately treated with the Baylis-Hillman adducts 5a-f,
leading in good yield to the acyl carbamates 6,7 as nearly equimolar diastereomeric mixtures. Since
these compounds were hard to separate, only small samples could be isolated for analytical purposes
and they were assigned as 6 or 7 on the basis of their Rf, exclusively (Scheme 2). Then, the mixtures of
acyl carbamates 6,7 were treated with DABCO [21], affording in moderate yield diastereomeric
mixtures of compounds 8 and 9 which in turn were easily separated by silica gel chromatography. The
configurations at the newly formed stereogenic centre were assigned as S for the major component of
the reaction mixture and as R for the minor one, on the basis of both mechanistic considerations and
1H-NMR data.

Molecules 2009, 14
Scheme 2. Synthesis of [2-(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1-carbox-
2826
amido)(aryl) methyl] acrylates 8 and 9.
O
R1
H
O
N
COOR2
O
R1
i
COOR2
N
N
O
4
+
OH
5a,f
6a-f,7a-f
ii
R1
R1
O
O
COOR2
COOR2
HN
HN
N
N
N
N
O
O
+
9a-e
8a-e
Reagents and Conditions: i. DCM, rt. (a) R1 = C6H5, R2 = CH3, 54%; (b) R1 = 4-Cl-C6H4,
R2 = C2H5, 50%; (c) R1 = 4-NO2-C6H4; R2 = CH3, 50%; (d) R1 = 1-naphthyl, R2 = C2H5, 60%; (e)
R1 = C2H5OOC, R2 = CH3, 77%; (f) R1 = (CH3)2CHCH2, R2 = CH3, 56%. ii. DABCO, DCM, r.t.
(a) R1 = C6H5, R2 = CH3, 60%, d.r. 75:25; (b) R1 = 4-Cl-C6H4, R2 = C2H5, 63%, d.r. 76:24;
(c) R1 = 4-NO2-C6H4; R2 = CH3, 61%, d.r. 70:30; (d) R1 = 1-naphthyl, R2 = C2H5, 58%, d.r. 70:30;
(e) R1 = C2H5OOC, R2 = CH3, 77%, d.r. 75:25; (f) R1 = (CH3)2CHCH2, R2 = CH3, only compound
3 was recovered in 80% yield.
Thus, having observed for the reaction leading to both 8 and 9 an interesting asymmetric induction,
that is attractive owing to the easy separation of the diastereomeric products 8 and 9, we first assigned
the configuration of the newly formed stereogenic centre on the basis of mechanistic considerations. In
fact, in the first step of the reaction of acylcarbamates 6,7, DABCO attacks the conjugate double bond
and the first products are the anionic species A and the cationic species B, both resulting from the
initial SN′ pathway, involving loss of carbon dioxide, irrespectively from the configuration at C-1' of
the starting acyl carbamate (Scheme 3).
Scheme 3. Formation of the intermediates A and B in the first SN' pathway.
CH3
N
O
N
O
CH3
O
R1
H
N
N
H
O
R1
- CO2
N
O
+
COOR2
H
N
O
NH2R3
A
B
COOR2
R3N
6,7
R3N = DABCO

Molecules 2009, 14
2827
The asymmetric induction arises from the second SN' pathway, in which the anion A attacks the
cationic species B leading to the ureas 8 and 9. As it appears from Scheme 4, the attack proceeding via
a pro-S mode is free from steric interactions, whereas in the attack occurring in the pro-R mode steric
interaction results from the phenyl group and B. Thus, the two transition states leading to 8 and 9,
respectively, are different in energy and the less demanding 8 is favoured with respect to 9.
Scheme 4. Preferential formation of compound 8 due to steric interaction in the second SN' pathway.
CH3
R1
N
O
H
N
O
H
COOR2
NR3
CH3
N
N
O
R1
N
O
H
H
N
COOR2
A
B
A
NR3
B
pro-S
mode
pro-R
mode
CH3
O
CH3
N
N
O
H
1
N
O
N
O
H
R
COOR2
N
N
H
H
COOR2
R1
S
R
8
9
Mechanistic considerations are supported also by the chemical shift pattern observed for the
olefinic protons for both 8 and 9. In fact, since H-1' in the preferential conformation must be co-planar
with the sp2 urea system, the C-3 olefinic protons in 8, displaying the S-configuration at carbon atom
of methine group attached to C-2, experience the shielding effect of the phenyl group of the auxiliary,
and are observed upfield with respect to the same protons in 9, having the R-configuration, where this
effect is missing. On the other hand, the chemical shift of H-1' remains unchanged in both
diastereomers (Table 1). Eventually, the configurational assignment at C-1' is in agreement with the Rf
values, observed for compounds 8 and 9, these latter being more polar owing to the less compact
conformation.
We can also tentatively explain the total lack of reactivity observed for the acylcarbamates 6f and
7f. In this case, the intermediate A surely forms, owing to the recover of compound 3, but further SN'
process cannot occur owing to the low electrophilicity of C-3, in which the alkyl group significantly
decreases the electron-withdrawing effect of both the ester and the ammonium ion, thus preventing the
second reaction.

Molecules 2009, 14
Table 1. Significant 1H-NMR data for both olefinic protons and H-1' in compounds 8 and 9.
2828
Compound δ(Hcis) δ(Htrans
)
δ(H-1')
5.26
5.28
5.26
5.27
5.26
5.28
5.28
5.32
5.26
5.28
8a
9a
8b
9b
8c
9c
8d
9d
8e
9e
6.29
6.36
6.31
6.37
6.38
6.43
6.38
6.46
6.31
6.37
5.82
5.91
5.81
5.89
5.92
5.97
5.90
6.00
5.85
5.92
In conclusion, starting from the Baylis-Hillman adducts 5, we were able to prepare chiral
derivatives of the aza-Baylis-Hillman adducts by using two successive SN' steps. These compounds can
be further elaborated by removing the chiral auxiliary, leading to monomers useful for preparing new
β-foldamers, and work along this line is currently underway.
Experimental
General
Melting points (uncorrected) were measured on a Electrothermal IA 9000 apparatus. NMR spectra
(200 MHz for 1H, 50 MHz for 13C, chemical shifts as ppm in the δ scale, coupling constants J in Hertz)
were recorded at 25 °C in CDCl3 solutions on a Varian Gemini 200 spectrometer. Diastereomeric
ratios were determined with a liquid chromatography Agilent Technologies HP1100 equipped with a
Zorbax Eclipse XDB-C8 Agilent Technologies column (flow rate 0.5 mL/min) and equipped with a
diode-array UV detector (220 and 254 nm). Enantiomeric purity of all compounds was determined
≥98% by using a Chiralcel OD column. Acetonitrile and methanol for HPLC were purchased from a
commercial supplier. Samples were prepared by diluting 1 mg in 5 mL of a 1:1 mixture of H2O and
acetonitrile in pure acetonitrile or in pure methanol. The MSD1100 mass detector was utilized under
the following conditions: mass range 100-2500 uma, positive scanning, energy of fragmentor 50 V,
drying gas flow (nitrogen) 10.0 mL/min, nebulizer pressure 45 psig, drying gas temperature 350 °C,
capillary voltage 4500 V. Optical rotations were measured on a Perkin Elmer 341 polarimeter. Column
chromatography was performed using Kieselgel 60 Merck (230-400 mesh ASTM).
(4S,5R)-1-(3,4-Dimethyl-2-oxo-5-phenylimidazolidine)carboxamide (3): The imidazolidin-2-one 1
(0.38 g; 2.0 mmol) was dissolved in dry THF (3 mL) and n-BuLi (0.74 mL of 2.7 M solution in
hexanes; 2.0 mmol) was added at 0 °C and after 1 h the solution was slowly added dropwise at -78 °C
to phosgene (1.98 mL of 1.98 M solution in toluene; 4.0 mmol) to give compound 2, which was not
isolated. After 3 h 30% aqueous NH4OH (1.2 mL) was added to the mixture which after 30 min was

Molecules 2009, 14
2829
extracted with DCM (2 × 40 mL). After drying (Na2SO4), the solvent was removed under reduced
pressure, to give compound 3 as a white solid (0.42 g; 90% yield), which was used without further
1
purification. H-NMR: δ 0.80 (d, J = 6.6 Hz, 3H, 4-CH3), 2.80 (s, 3H, 3-CH3), 3.91 (dq, J = 6.6 Hz,
J = 9.3 Hz, 1H, H-4), 4.99 (br s, 1H, NH), 5.27 (d, J = 9.3 Hz, 1H, H-5), 7.13 - 7.17 (m, 2 ArH), 7.22 -
13
7.38 (m, 3 ArH) 8.11 (br s, 1H, NH); C-NMR: δ 14.8, 28.2, 54.5, 59.1, 126.8, 128.1, 128.5, 136.7,
153.1 157.4; [α]D -3.7 (c 0.5, CHCl3) (Lit.[19]: -2.3 c 1, CH2Cl2); MS (ESI): m/z 233.1 [M]+, 256.1
[M+Na]+; Anal. calcd. for C12H15N3O2: C, 61.79; H, 6.48; N, 18.01. Found: C, 61.74; H, 6.54;
N, 17.96.
Preparation of derivatives 6 and 7
To a solution containing compound 3 (1.17 g; 5.0 mmol) in dichloroethane (10 mL), oxalyl chloride
(0.66 g; 5.2 mmol) was added and the mixture was refluxed for 4 h. The solvent was partially removed
and then the Baylis-Hillman adducts 5a-f (3.3 mmol) were added, dissolved in DCM (10 mL). The
reaction was stirred for 12 h, volatiles were removed under reduced pressure and the products 6 and 7
were obtained as equimolar mixtures hard to separate by silica gel chromatography (cyclohexane-ethyl
acetate 1:1), although small amounts could be obtained pure enough for analytical determinations.
Methyl(1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carbonylcarbamoyloxy)-
(phenyl)methyl]acrylate (6a) and its (1'R,4"S,5"R)-isomer 7a: The title compounds were obtained in
54% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z 451.2 [M+],
474.2 [M+Na]+; Anal. Calcd. for C24H25N3O6: C, 63.85; H, 5.58; N, 9.31; Found: C, 63.79; H, 5.54; N,
1
9.35. Isomer 6a: Rf 0.63 (cyclohexane-AcOEt 1:1); H-NMR: δ 0.82 (d, J = 6.6 Hz, 3H, 4"-CH3),
2.82 (s, 3H, 3"-CH3), 3.68 (s, 3H, OCH3), 3.95 (dq, J = 6.6 Hz, J = 8.7 Hz, 1H, H-4"), 5.29 (d, J = 8.7
Hz, 1H, H-5"), 5.97 (bs, 1H, H-1'), 6.41 (s, 1H, =CH2), 6.67 (s, 1H, =CH2), 7.07 - 7.18 (m, 2 ArH),
7.24 - 7.42 (m, 8 ArH), 10.98 (s, 1H, NH); 13C-NMR: δ 14.5, 28.0, 51.9, 54.3, 59.0, 74.4, 126.2, 126.8,
126.9, 127.7, 127.8, 128.3, 128.5, 128.7, 135.6, 137.3, 139.0, 147.2, 149.3, 156.8, 165.2. Isomer 7a: Rf
1
0.58 (cyclohexane-AcOEt 1:1); H-NMR: δ 0.82 (d, J = 6.6, 3H, 4"-CH3), 2.84 (s, 3H, 3"-CH3), 3.68
(s, 3H, OCH3), 3.96 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.31 (d, J = 8.8 Hz, 1H, H-5"), 5.97 (bs s,
1H, H-1'), 6.41 (s, 1H, =CH2), 6.68 (s, 1H, =CH2), 7.07 - 7.18 (m, 2 ArH), 7.23 - 7.44 (m, 8 ArH),
13
10.97 (s, 1H, NH); C-NMR: δ 14.6, 28.0, 51.9, 54.3, 59.1, 74.4, 126.0, 126.8, 126.9, 127.8, 128.4,
128.5, 128.6, 135.6, 139.0, 147.3, 149.3, 156.9, 165.2.
Ethyl(1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carbonylcarbamoy-loxy)(4-
chlorophenyl)methyl]acrylate (6b) and its (1'R,4"S,5"R)-isomer 7b: The title compounds were
obtained in 50% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z
499.2 [M]+, 522.2 [M+Na]+; Anal. Calcd. for C25H26ClN3O6: C, 60.06; H, 5.24; N, 8.40; Found: C,
60.02; H, 5.19; N, 8.35. Isomer 6b: Rf 0.67 (cyclohexane-AcOEt 1:1); 1H-NMR: δ 0.82 (d, J = 6.7 Hz,
3H, 3"-CH3), 1.19 (t, J = 7.2 Hz, 3H, CH3CH2O), 2.83 (s, 3H, 3"-CH3), 3.96 (dq, J = 6.7 Hz,
J = 8.8 Hz, 1H, H-4"), 4.13 (q, J = 7.2 Hz, 2H, CH3CH2O), 5.29 (d, J = 8.8 Hz, 1H, H-5"), 5.98 (s, 1H,
H-1'), 6.42 (s, 1H, =CH2), 6.62 (s, 1H, =CH2), 7.07 - 7.17 (m, 2 ArH), 7.21 - 7.86 (m, 8 ArH), 11.00 (s,
1H, NH); 13C-NMR: δ 13.9, 14.6, 28.0, 54.4, 59.1, 61.0, 73.9, 126.0, 126.9, 128.4, 128.5, 128.6, 129.3,

Molecules 2009, 14
2830
134.3, 135.6, 136.1, 136.9, 147.2, 149.2, 156.9, 164.6. Isomer 7b: Rf 0.61 (cyclohexane-AcOEt 1:1);
1H-NMR: δ 0.83 (d, J = 6.6 Hz, 3H, 3"-CH3), 1.23 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.84 (s, 3H, 3"-
CH3), 3.97 (dq, J = 6.6 Hz, J = 8.7 Hz, 1H, H-4"), 4.13 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.30 (d, J = 8.7
Hz, 1H, H-5"), 5.97 (s, 1H, H-1'), 6.41 (s, 1H, =CH2), 6.63 (s, 1H, =CH2), 7.05 - 7.17 (m, 2 ArH), 7.21
- 7.38 (m, 8 ArH), 10.99 (s, 1H, NH); 13C-NMR: δ 14.0, 14.6, 28.1, 54.4, 59.2, 61.0, 73.9, 125.9,
126.9, 128.4, 128.6, 129.4, 134.4, 135.6, 136.0, 139.0, 147.3, 149.3, 156.9, 164.6.
Methyl (1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carbonylcarbamoyloxy)-
(4-nitrophenylmethyl)]acrylate (6c) and its (1'R,4"S,5"R)-isomer (7c): The title compounds were
obtained in 50% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z
496.2 [M]+, 519.2 [M+Na]+; Anal. Calcd. for C24H24N4O8: C, 58.06; H, 4.87; N, 11.28; Found: C,
1
58.00; H, 4.84; N, 11.33. Isomer 6c: Rf 0.56 (cyclohexane-AcOEt 1:1); H-NMR: δ 0.83 (d, J = 6.6
Hz, 3H, 4"-CH3), 2.84 (s, 3H, 3"-CH3), 3.69 (s, 3H, OCH3), 3.97 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-
4"), 5.30 (d, J = 8.8 Hz, 1H, H-5"), 6.08 (d, J = 1.3 Hz, 1H, H-1'), 6.47 (s, 1H, =CH2), 6.70 (s, 1H,
=CH2), 7.08 - 7.18 (m, 2 ArH), 7.25 - 7.35 (m, 3 ArH), 7.58 (d, J = 8.9 Hz, 2 ArH), 8.16 (d, J = 8.9 Hz,
13
2 ArH), 11.09 (s, 1H, NH); C-NMR: δ 14.6, 26.9, 28.1, 52.1, 54.4, 59.2, 73.5, 123.7, 126.9, 127.3,
128.5, 128.7, 135.6, 138.1, 144.7, 147.2, 147.9, 149.2, 156.9, 164.8. Isomer 7c: Rf 0.49 (cyclohexane-
AcOEt 1:1); 1H-NMR: δ 0.83 (d, J = 6.6 Hz, 3H, 4"-CH3), 2.84 (s, 3H, 3"-CH3), 3.69 (s, 3H, OCH3),
3.98 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.30 (d, J = 8.8 Hz, 1H, H-5"), 6.06 (d, J = 1.1 Hz, 1H, H-
1'), 6.46 (s, 1H, =CH2), 6.71 (s, 1H, =CH2), 7.08 - 7.18 (m, 2 ArH), 7.21 - 7.35 (m, 3 ArH), 7.58 (d,
J = 8.8 Hz, 2 ArH), 8.17 (d, J = 8.8 Hz, 2 ArH), 11.09 (s, 1H, NH); 13C-NMR: δ 14.6, 26.9, 28.1, 52.1,
54.4, 59.2, 73.5, 123.7, 126.8, 126.9, 127.2, 127.8, 128.1, 128.5, 128.7, 135.5, 138.1, 144.6, 147.2,
147.8, 149.2, 156.9, 164.8.
Ethyl
(1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carbonylcarbamoyloxy)-
(naphthalen-1-ylmethyl)]acrylate (6d) and its (1'R,4"S,5"R)-isomer (7d): The title compounds were
obtained in 60% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z
515.2 [M]+, 538.2 [M+Na]+; Anal. Calcd. for C29H29N3O6: C, 67.56; H, 5.67; N, 8.15; Found: C,
67.51; H, 5.62; N, 8.19. Isomer 6d: Rf 0.62 (cyclohexane-AcOEt 1:1); 1H-NMR: δ 0.80 (d, J = 6.6 Hz,
3H, 4"-CH3), 1.17 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.81 (s, 3H, 3"-CH3), 3.92 (dq, J = 6.6 Hz, J = 8.8
Hz, 1H, H-4"), 4.12 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.28 (d, J = 8.8 Hz, H-5"), 6.02 (d, J = 1.3 Hz, 1H,
H-1'), 6.46 (s, 1H, =CH2), 6.85 (s, 1H, =CH2), 7.09 - 7.15 (m, 2 ArH), 7.21 - 7.38 (m, 3ArH), 7.40 -
13
7.51 (m, 3ArH), 7.74 - 7.87 (m, 4ΑrΗ), 11.02 (s, 1H, NH); C-NMR: δ 14.0, 14.6, 26.9, 28.0, 54.4,
59.1, 60.9, 74.7, 125.3, 126.1, 126.3, 127.0, 127.4, 127.6, 128.1, 128.2, 128.4, 128.6, 133.0, 133.3,
1
134.8, 135.7, 139.3, 147.3, 149.4, 156.9, 164.8. Isomer 7d: Rf 0.56 (cyclohexane-AcOEt 1:1); H-
NMR: δ 0.76 (d, J = 6.6 Hz, 3H, 4"-CH3), 1.18 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.79 (s, 3H, 3"-CH3),
3.90 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 4.12 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.28 (d, J = 8.8 Hz,
1H, H-5"), 6.05 (s, 1H, H-1'), 6.47 (s, 1H, =CH2), 6.88 (s, 1H, =CH2), 7.05 -7.16 (m, 2 ArH), 7.20 -
7.32 (m, 3 ArH), 7.40 - 7.55 (m, 3 ArH), 7.75 - 7.91 (m, 4 ArH), 11.07 (s, 1H, NH); 13C-NMR: δ 13.8,
14.4, 26.8, 27.9, 54.2, 59.0, 60.8, 74.6, 125.2, 125.8, 126.0, 126.2, 126.8, 127.3, 127.5, 128.1, 128.2,
128.4, 132.9, 133.2, 134.6, 135.6, 139.2, 147.2, 149.3, 156.7, 164.6.

Molecules 2009, 14
2831
Methyl (1'R,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carbonylcarbamoyloxy)-
(ethoxycarbonylmethyl]acrylate (6e) and its (1'S,4"S,5"R)-isomer (7e): The title compounds were
obtained in 77% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z
447.2 [M]+, 470.2 [M+Na]+; Anal. Calcd. for C21H25N3O8: C, 56.37; H, 5.63; N, 9.39; Found: C,
1
56.33; H, 5.57; N, 9.43. Isomer 6e: Rf 0.57 (cyclohexane-AcOEt 1:1); H-NMR: δ 0.80 (d, J = 6.6,
3H, 4"-CH3), 1.21 (t, J = 7.0 Hz, 3H, CH3CH2O), 2.80 (s, 3H, 3"-CH3), 3.77 (s, 3H, OCH3), 3.93 (dq,
J = 6.6, J = 8.7, 1 H, H-4"), 4.17 (q, J = 7.0 Hz, 2H, CH3CH2O), 5.29 (d, J = 8.7, 1H, H-5"), 5.94 (s,
1H, H-1'), 6.00 (s, 1H, =CH2), 6.47 (s, 1H, =CH2), 7.06 – 7.21 (m, 3 ArH), 7.22 – 7.93 (m, 7 ArH +
NH); + NH); 13C-NMR:: δ 14.5, 28.0, 51.9, 54.3, 59.0, 74.4, 126.2, 126.9, 127.7, 127.8, 128.3, 128.5,
128.7, 135.6, 137.3, 139.0, 147.2, 149.3, 156.8, 165.2. Isomer 7e: Rf 0.51 (cyclohexane-AcOEt 1:1);
1H-NMR: δ 0.83 (d, J = 6.6, 3H, 4"-CH3), 1.22 (t, J = 7.0 Hz, 3H, CH3CH2O), 2.81 (s, 3H, 3"-CH3),
3.76 (s, 3H, OCH3), 3.95 (dq, J = 6.6, J = 8.8, 1H, H-4"), 4.19 (q, J = 7.0 Hz, 2H, CH3CH2O), 5.31 (d,
J = 8.8, 1H, H-5"), 5.95 (s, 1H, H-1'), 5.99 (s, 1H, =CH2), 6.48 (s, 1H, =CH2), 7.07 – 7.20 (m, 3 ArH),
13
7.26 – 7.47 (m, 7 ArH + NH); C-NMR: δ 14.6, 28.0, 51.9, 54.3, 59.1, 74.4, 126.0, 126.8, 126.9,
127.8, 128.4, 128.5, 128.6, 135.6, 139.0, 147.3, 149.3, 156.8, 165.2.
Methyl
(3S,4'S,5'R)-3-(3',4'-dimethyl-2'-oxo-5'-phenylimidazolidine-1'-carbonylcarbamoyloxy)-5-
methyl-2-methylenehexanoate (6f) and its (3R,4'S,5'R)-isomer (7f): The title compounds were obtained
in 56% overall yield as an equimolar diastereomeric mixture. White solid; MS (ESI): m/z 431.2 [M]+,
454.2 [M+Na]+; Anal. Calcd. for C22H29N3O6: C, 61.24; H, 6.77; N, 9.74. Found: C, 61.18; H, 6.73; N,
9.69. Isomer 6f: Rf 0.53 (cyclohexane-AcOEt 1:1); 1H-NMR: δ 0.81 (d, J = 6.6, 3H, CH3), 0.90 (d,
J = 6.6, 3H, CH3), 0.93 (d, J = 6.6, 3H, 4'-CH3), 1.45 – 1.73 (m, 3H, CH-CH2), 2.83 (s, 3H, 3'-CH3),
3.73 (s, 3H, OCH3), 3.96 (dq, J = 6.6, J = 8.8, 1H, H-4'), 5.30 (d, J = 8.8, 1H, H-5'), 5.66 (dd, J = 4.0,
J = 8.8, 1H, H-3), 5.82 (s, 1H, =CH2), 6.25 (s, 1H, =CH2), 7.09 – 7.18 (m, 3 ArH), 7.26 – 7.35 (m, 2
13
ArH + NH); C NMR (50 MHz, CDCl3) δ 14.5, 21.6, 23.1, 24.7, 26.8, 29.1, 43.7, 51.8, 54.3, 59.1,
72.0, 125.3, 126.9, 128.3, 128.5, 135.7, 140.3, 147.3, 149.7, 156.9, 165.5. Isomer 7f: Rf 0.44
(cyclohexane-AcOEt 1:1); 1H-NMR: δ 0.79 (d, J = 6.6, 3H, CH3), 0.88 (d, J = 5.3, 3H, CH3), 0.91 (d,
J = 5.3, 3H, 4'-CH3), 1.41 - 1.71 (m, 3H, CH-CH2), 2.81 (s, 3H, 3'-CH3), 3.73 (s, 3H, OCH3), 3.95 (dq,
J = 5.3, J = 8.8, 1H, H-4'), 5.28 (d, J = 8.8, 1H, ), 5.65 (dd, J = 3.3, J = 8.9, 1H, H-3), 5.79 (s, 1H,
=CH2), 6.24 (s, 1H, =CH2), 7.07 – 7.15 (m, 3 ArH), 7.23 – 7.35 (m, 2 ArH + NH); 13C-NMR: δ 14.6,
21.5, 23.1, 24.6, 26.8, 27.9, 43.7, 51.7, 54.3, 59.0, 71.8, 125.0, 126.9, 128.2, 128.5, 135.7, 140.4,
147.3, 149.6, 158.9, 165.5.
Preparation of compounds 8 and 9
To a solution containing an equimolar mixture of acyl carbamates 6 and 7 (3.0 mmol) in dry DCM
(8.0 mL), DABCO (0.07 g; 0.6 mmol) was slowly added at r.t. After 20 min, 2 M HCl (10 mL) was
added and the mixture was extracted with DCM (2 × 35 mL). After drying (Na2SO4) and removal of
the volatiles, the residue was purified by silica gel chromatography (cyclohexane:ethyl acetate 60:40),
to give pure compounds 8 and 9.

Molecules 2009, 14
Methyl (1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carboxamido)(phenyl)-
2832
methyl]acrylate (8a) and its (1'R,4"S,5"R)-isomer (9a): The title compounds were obtained in 60%
overall yield as 75:25 diastereomeric mixture. MS (ESI): m/z 407.2 [M]+, 430.2 [M+Na]+; Anal. Calcd.
for C23H25N3O4: C, 67.80; H, 6.18; N, 10.31; Found: C, 67.84; H, 6.13; N, 10.27. Isomer 8a: Rf 0.47
(cyclohexane-AcOEt 1:1); White crystals; Mp 50-52 °C; 1H-NMR: δ 0.80 (d, J = 6.6 Hz, 3H, 4"-CH3),
2.80 (s, 3H, 3"-CH3), 3.64 (s, 3H, OCH3), 3.91 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.26 (d, J = 8.7
Hz, 1H, H-1'), 5.82 (s, 1H, =CH2), 5.89 (d, J = 8.8 Hz, 1H, H-5"), 6.29 (s, 1H, =CH2), 7.11 - 7.19 (m, 2
ArH), 7.20 - 7.41 (m, 8 ArH), 9.21 (d, J = 8.7, 1H, NH); 13C-NMR: δ 14.7, 26.9, 28.0, 51.8, 54.5, 54.8,
59.3, 125.7, 126.8, 126.9, 127.4, 128.0, 128.5, 136.8, 140.1, 140.3, 151.6, 157.9, 165.9; [α]D -122.0 (c
0.5, CHCl3). Isomer 9a: Rf 0.27 (cyclohexane-AcOEt 1:1); White crystals; Mp 123-125 °C; 1H-NMR:
δ 0.81 (d, J = 6.6 Hz, 3H, 4"-CH3), 2.81 (s, 3H, 3"-CH3), 3.66 (s, 3H, OCH3), 3.94 (dq, J = 6.6 Hz,
J = 8.8 Hz, 1H, H-4"), 5.28 (d, J = 8.5 Hz, 1H, H-1'), 5.89 (d, J = 8.8 Hz, 1H, H-5"), 5.91 (s, 1H,
=CH2), 6.36 (s, 1H, =CH2), 7.08 - 7.17 (m, 2 ArH), 7.20 - 7.39 (m, 8 ArH), 9.15 (d, J = 8.5 Hz, 1H,
13
NH); C- NMR: δ 14.8, 26.9, 28.0, 51.8, 54.6, 54.9, 59.4, 126.1, 126.7, 126.8, 127.0, 127.3, 127.8,
128.1, 128.3, 128.5, 128.6, 136.8, 139.6, 140.6, 151.8, 157.9, 165.9; [α]D 93.0 (c 0.5, CHCl3).
Ethyl
(1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carboxamido)(4-chloro-
phenyl)methyl]acrylate (8b) and its (1'R,4"S,5"R)-isomer (9b): The title compounds were obtained in
63% overall yield as 76:24 diastereomeric mixture. MS (ESI): m/z 455.2 [M] +, 478.2 [M+Na]+; Anal.
Calcd. for C24H26ClN3O4: C, 63.22; H, 5.75; N, 9.22; Found: C, 63.17; H, 5.71; N, 9.26. Isomer 8b: Rf
0.47 (cyclohexane-AcOEt 1:1); White crystals; Mp 46-48 °C; 1H-NMR: δ 0.80 (d, J = 6.6 Hz, 3H, 4"-
CH3), 1.16 (t, J = 7.2 Hz, 3H, CH3CH2O), 2.80 (s, 3H, 3"-CH3), 3.94 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H,
H-4"), 4.09 (50% q, J = 7.2 Hz, 2H, CH3CH2O), 4.10 (50% q, J = 7.2 Hz, 2H, CH3CH2O), 5.26 (d,
J = 8.3 Hz, 1H, H-1'), 5.81 (s, 1H, =CH2), 5.86 (d, J = 8.8 Hz, 1H, H-5"), 6.31 (s, 1H, =CH2), 7.10 -
7.18 (m, 2 ArH), 7.22 - 7.38 (m, 7 ArH), 9.22 (d, J = 8.3 Hz, 1H, NH); 13C-NMR: δ 13.9, 14.8, 26.9,
28.1, 54.4 (50%), 54.6 (50%), 59.3, 61.0, 125.9, 126.8, 128.1, 128.3, 128.4, 128.5, 128.6, 133.2, 136.8,
139.0, 140.2, 151.7, 157.9, 165.3; [α]D -125.0 (c 0.5, CHCl3). Isomer 9b: Rf 0.29 (cyclohexane-
1
AcOEt 1:1); Low melting white solid; H-NMR: δ 0.80 (d, J = 6.6 Hz, 3H, 4"-CH3), 1.17 (t, J = 7.2
Hz, 3H, CH3CH2O), 2.80 (s, 3H, 3"-CH3), 3.93 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 4.10 (q, J = 7.2
Hz, 3H, CH3CH2O), 5.26 (d, J = 8.8 Hz, 1H, H-1'), 5.86 (d, 1H, J = 8.8 Hz, 1H, H-5"), 5.89 (s, 1H,
=CH2), 6.37 (s, 1H, =CH2), 7.09 - 7.18 (m, 2 ArH), 7.19 - 7.38 (m, 7 ArH), 9.17 (d, J = 8.8, 1H, NH);
13C-NMR: δ 13.9, 14.8, 28.0, 29.7, 54.4 (50%), 54.6 (50%), 59.4, 60.9, 126.4, 126.8, 128.1, 128.2,
128.5, 133.1, 136.8, 138.5, 140.3, 151.8, 157.9, 165.3; [α]D 155.0 (c 0.5, CHCl3).
Methyl
(1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carboxamido)(4-nitro-
phenyl)methyl]acrylate (8c) and its (1'R,4"S,5"R)-isomer (9c): The title compounds were obtained in
61% overall yield as 70:30 diastereomeric mixture. MS (ESI): m/z 452.2 [M]+, 475.2 [M+Na]+; Anal.
Calcd. for C23H24N4O6: C, 61.05; H, 5.35; N, 12.38; Found: C, 61.00; H, 5.31; N, 12.35. Isomer 8c: Rf
0.50 (cyclohexane-AcOEt 1:1); White crystals; Mp. 55-57 °C; 1H-NMR: δ 0.82 (d, J = 6.6 Hz, 3H, 4"-
CH3), 2.83 (s, 3H, 3"-CH3), 3.68 (s, 3H, OCH3), 3.95 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.26 (d,
J = 8.7 Hz, 1H, H-1'), 5.92 (s, 1H, =CH2), 5.95 (d, J = 8.8 Hz, 1H, H-5"), 6.38 (s, 1H, =CH2), 7.10 -
7.22 (m, 2 ArH), 7.25 - 7.41 (m, 3 ArH), 7.51 (d, J = 8.5 Hz, 2 ArH), 8.16 (d, J = 8.5 Hz, 2 ArH), 9.44

Molecules 2009, 14
2833
13
(d, J = 8.7 Hz, 1H, NH); C-NMR: δ 14.8, 28.1, 52.1 (50%), 52.2 (50%), 54.6 (50%), 54.8 (50%),
59.4, 76.4, 123.8, 126.8, 127.5, 127.8, 128.2, 128.6, 136.7, 139.1, 147.8, 151.9, 157.9, 165.5; [α]D -
107.0 (c 0.5, CHCl3). Isomer 9c: Rf 0.20 (cyclohexane-AcOEt 1:1); White crystals; Mp. 62-64 °C; 1H-
NMR: δ 0.83 (d, J = 6.6 Hz, 3H, 4"-CH3), 2.83 (s, 3H, 3"-CH3), 3.70 (s, 3H, OCH3), 3.96 (dq,
J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.28 (d, J = 8.8 Hz, 1H, H-1'), 5.97 (s, 1H, =CH2), 5.97 (d, J = 8.5
Hz, 1H, H-5"), 6.43 (s, 1H, =CH2), 7.11 - 7.18 (m, 2 ArH), 7.25 - 7.38 (m, 3 ArH), 7.44 (d, J = 8.5 Hz,
2 ArH), 8.13 (d, J = 8.5 Hz, 2 ArH), 9.37 (d, J = 8.8 Hz, 1H, NH); 13C-NMR: δ 14.8, 28.1, 52.1, 54.6
(50%), 54.8 (50%), 59.5, 76.4, 123.7, 126.8, 127.4, 128.0, 128.3, 128.6, 136.7, 139.3, 147.4, 152.0,
156.2 (50%), 157.8 (50%), 165.4; [α]D 78.9 (c 0.5, CHCl3).
Ethyl (1'S,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carboxamido) (naphthalen-
1-yl)methyl]acrylate (8d) and its (1'R,4"S,5"R)-isomer (9d): The title compounds were obtained in
58% overall yield as 70:30 diastereomeric mixture. MS (ESI): m/z 471.2 [M]+, 494.2 [M+Na]+; Anal.
Calcd. for C28H29N3O4: C, 71.32; H, 6.20; N, 8.91. Found: C, 71.36; H, 6.15; N, 8.87. Isomer 8d: Rf
1
0.50 (cyclohexane-AcOEt 1:1); White crystals; Mp. 53-55 °C; H-NMR: δ 0.79 (d, J = 6.6 Hz, 3H, 4"-
CH3), 1.13 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.79 (s, 3H, 3"-CH3), 3.88 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H,
H-4"), 4.08 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.28 (d, J = 8.5 Hz, 1H, H-1'), 5.90 (s, 1H, =CH2), 6.10 (d,
J = 8.8 Hz, 1H, H-5"), 6.38 (s, 1H, =CH2), 7.15 - 7.21 (m, 2 ArH), 7.25 - 7.52 (m, 6 ArH), 7.74 - 7.86
13
(m, 4 ArH), 9.31 (d, J = 8.5, 1H, NH); C-NMR: δ 13.9, 14.7, 28.0, 54.5, 55.0, 59.3, 60.8, 125.3,
125.6, 125.7, 125.8, 126.0, 126.8, 127.4, 127.6, 127.7, 128.0, 128.3, 128.5, 132.8, 133.4, 137.0, 137.8,
140.6, 151.7, 157.9, 165.5; [α]D -106.0 (c 0.5, CHCl3). Isomer 9d: Rf 0.35 (cyclohexane-AcOEt 1:1);
1
White crystals; Mp. 56-57 °C; H-NMR: δ 0.79 (d, J = 6.6 Hz, 3H, 4"-CH3), 1.16 (t, J = 7.1 Hz, 3H,
CH3CH2O), 2.80 (s, 3H, 3"-CH3), 3.91 (dq, J = 6.6 Hz, J = 8.8 Hz, 1H, H-4"), 5.32 (d, J = 8.8 Hz, 1H,
H-1'), 6.00 (s, 1H, =CH2), 6.11 (d, J = 8.7 Hz, 1H, H-5"), 6.46 (s, 1H, =CH2), 7.14 - 7.20 (m, 2 ArH),
7.24 - 7.33 (m, 3 ArH), 7.39 - 7.49 (m, 3 ArH), 7.73 - 7.84 (m, 4 ArH), 9.29 (d, J = 8.7 Hz, 1H, NH);
13C-NMR: δ 13.8, 4.6, 27.9, 54.4, 54.8, 59.3, 60.7, 124.9, 125.4, 125.7, 125.9, 126.0, 126.7, 127.4,
127.9, 128.1, 128.4, 128.6, 132.6, 133.2, 136.8, 137.2, 140.7, 151.8 157.8, 165.4; [α]D 54.0
(c 0.5, CHCl3).
Methyl (1'R,4"S,5"R)-2-[(3",4"-dimethyl-2"-oxo-5"-phenylimidazolidine-1"-carboxamido) (ethoxy-
carbonyl)methyl]acrylate (8e) and its (1'S,4"S,5"R)-isomer (9e): The title compounds were obtained in
77% overall yield as 75:25 diastereomeric mixture. MS (ESI): m/z 403.2 [M]+, 426.2 [M+Na]+; Anal.
Calcd. for C20H25N3O6: C, 59.54; H, 6.25; N, 10.42; Found: C, 59.50; H, 6.21; N, 10.46. Isomer 8e: Rf
0.35 (cyclohexane-AcOEt 1:1); White crystals; Mp. 78-80 °C; 1H-NMR: δ 0.78 (d, J = 6.6 Hz, 3H, 4"-
CH3), 1.21 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.80 (s, 3H, 3"-CH3), 3.78 (s, 3H, OCH3), 3.88 (dq, J = 6.6
Hz, J = 8.4 Hz, 1H, H-4"), 4.15 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.26 (d, J = 8.7 Hz, 1H, H-1'), 5.33 (d,
J = 8.4 Hz, 1H, H-5"), 5.85 (s, 1H, =CH2), 6.31 (s, 1H, =CH2), 7.09 - 7.18 (m, 2 ArH), 7.22 - 7.35 (m,
3 ArH), 9.22 (d, J = 8.7 Hz, 1H, NH); 13C-NMR: δ 14.0, 14.7, 28.1, 52.1, 54.5, 54.6, 59.2, 61.7, 126.8,
128.0, 128.4, 129.3, 136.5, 136.8, 152.2, 157.6, 165.5, 169.7; [α]D 17.8 (c 0.5, CHCl3). Isomer 9e: Rf
1
0.25 (cyclohexane-AcOEt 1:1); Low melting white solid; H-NMR: δ 0.80 (d, J = 6.6 Hz, 3H, 4"-
CH3), 1.21 (t, J = 7.1 Hz, 3H, CH3CH2O), 2.81 (s, 3H, 3"-CH3), 3.79 (s, 3H, OCH3), 3.91 (dq, J = 6.6
Hz, J = 8.8 Hz, 1H, H-4"), 4.18 (q, J = 7.1 Hz, 2H, CH3CH2O), 5.23 (d, J = 8.8 Hz, 1H, H-5"), 5.28 (d,

Molecules 2009, 14
2834
J = 8.2 Hz, 1H, H-1'), 5.92 (s, 1H, =CH2), 6.37 (s, 1H, =CH2), 7.10 - 7.21 (m, 2 ArH), 7.25 - 7.38 (m, 3
13
ArH), 9.25 (d, J = 8.2 Hz, 1H, NH); C-NMR: δ 14.0, 14.8, 28.1, 52.1, 54.5, 54.8, 59.4, 61.8, 126.9,
128.1, 128.5, 129.6, 136.8, 152.1, 157.6, 165.5, 169.5; [α]D - 41.7 (c 0.5, CHCl3).
Acknowledgements
We thank Diatech s.r.l. and Nanodream s.r.l., Iesi (Ancona, Italy) for financial support.
References and Notes
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Sample Availability: Samples of all the compounds are available from the authors.
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
This article is an open-access article distributed under the terms and conditions of the Creative
Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).