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L.-W. Zheng et al. / Bioorg. Med. Chem. Lett. 21 (2011) 3909–3913
Figure 4. Morphology image of A549 (A–D) and H322 (E–H) cells treated with the compounds 3a, 3b or 3i for 48 h (200Â). A and E are control, the cells treated with DMSO
0.1% (v/v) as a vehicle control. B and F, C and G, D and H are treated with the compounds 3a, 3b or 3i at concentration of 40 lM respectively.
Acknowledgment
This research was supported by National Natural Science Foun-
dation of China (90813022 and 20972088).
Supplementary data
Supplementary data associated with this article can be found, in
References and notes
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Figure 5. Effects of the compounds 3a, 3b or 3i on LDH release in A549 cells (A) and
H322 cells (B). A549 and H322 cells were treated with compounds 3a, 3b or 3i at
concentration of 40 lM or left untreated (control) for 48 h. LDH assay results are
presented as mean SE (n = 3).
microwave-assisted condition. Application of microwave irradia-
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experimental procedure is operationally easy and leads to high
yields in short reaction time without using toxic reagents and sol-
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compound 3i by X-ray analysis. The overall crystal structures were
stabilized through intra- and intermolecular hydrogen bonds and
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C–HÁÁÁ
p networks. Preliminary biological evaluation showed that
the compounds 3a, 3b and 3i could inhibit the growth of A549
and H322 cells in dosage-dependent manners and the compounds
have more inhibitory effect on A549 cells than H322 cells.
25. Experimental procedure and a typical structure elucidation: To a flask ethyl 1-(2-
oxo-2-phenylethyl)-3-phenyl-1H-pyrazole-5-carboxylate
derivatives
1