OPTIMIZATION OF ENZYME CATALYZED PEPTIDE SYNTHESIS IN A "WATER - WATER-IMMISCIBLE ORGANIC SOLVENT" BIPHASIC SYSTEM

Article abstract of DOI:10.1016/S0040-4020(01)98818-0

Optimal conditions were found for enzymatic synthesis of the dipeptide, N-acetyl-L-tryptophanyl-L-leucine amide in the biphasic system water - ethyl acetate.The synthesis was carried out using both free and immobilized α-chymotrypsin.Optimization was performed by such parameters as the "organic phase/aqueous phase" volume ratio, the pH of aqueous phase, and the concentration of starting reactants.Under most favourable conditions the dipeptide was synthesized on the preparative scale in ca. 100percent yield.As a result of immobilization (adsorption on the Sorsilen terephtalate support) the enzyme practically did not inactivate and may be used repeatedly.