Enantioselective synthesis of ε-lactones by lipase-catalyzed resolution

Article abstract of DOI:10.3987/COM-12-12426

Synthesis of optically active ε-dodecalactone (1) by lipase-catalyzed enantioselective acylation with racemic N-alkyl-6-hydroxydodecanamide (rac-2) as a substrate was attempted. Lipase PS-catalyzed acetylation using rac-2 progressed efficiently, and both

Full text of DOI:10.3987/COM-12-12426

HETEROCYCLES, Vol. 85, No. 5, 2012
1061
HETEROCYCLES, Vol. 85, No. 5, 2012, pp. 1061 - 1075. © 2012 The Japan Institute of Heterocyclic Chemistry
Received, 12th January, 2012, Accepted, 19th March, 2012, Published online, 30th March, 2012
DOI: 10.3987/COM-12-12426
ENANTIOSELECTIVE
SYNTHESIS
OF
ε-LACTONES
BY
LIPASE-CATALYZED RESOLUTION
Yasutaka Shimotori,a,* Masakazu Aoyama,a Hidetaka Tsukasa,b and Tetsuo
Miyakoshic
a) Department of Biotechnology and Environmental Chemistry, Kitami Institute
of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan; E-mail:
yasu@mail.kitami-it.ac.jp
b) Toyotama International Inc., 1-14-5 Kakigara-cho, Nihonbashi, Tokyo
103-0014, Japan
c) Department of Applied Chemistry, School of Science and Technology, Meiji
University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki 214-8571, Japan
Abstract – Synthesis of optically active ε-dodecalactone (1) by lipase-catalyzed
enantioselective acylation with racemic N-alkyl-6-hydroxydodecanamide (rac-2)
as a substrate was attempted. Lipase PS-catalyzed acetylation using rac-2
progressed efficiently, and both enantiomers of 1 were obtained with over 90%
optical purities.
INTRODUCTION
A cyclic ester moiety constitutes a frequently encountered structural motif in a large variety of natural
products and biologically active compounds. Lactone functionality exists in common flavor
components and is employed in the perfumery and food industries.1,2 However, the existence of
ε-lactones in natural products has not been reported. ε-Decalactone has a nut-like note and
ε-dodecalactone has a peach-like note, and they have been used as food flavorings. It is also
well-known that both enantiomers of γ- and δ-lactones have different odors and tastes.3,4 It is
conceivable that ε-lactones also show these differences, but they have not been reported. However,
several methods of synthesizing optically active ε-lactones have been reported.5-8 Pchelka et al.
synthesized (S)-ε-decalactone [(S)-1’] and (S)-ε-dodecalactone [(S)-1] by lipase-catalyzed
enantioselective perhydrolysis resulted from Baeyer-Villiger oxidation of racemic 1-alkylcyclohexanone,9

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HETEROCYCLES, Vol. 85, No. 5, 2012
but (S)-1’ and (S)-1 were synthesized with only 72% and 57% optical purities, respectively. Fellous et
al. synthesized (R)-1’ and (R)-1 by esterase-catalyzed enantioselective hydrolysis of racemic 1’ and 1
with optical purities of 88% and 33%, respectively.10 (R)-1’ had high optical purity, but it can hardly be
said that (R)-1 did. In this study, we attempted to synthesize optically active ε-lactones (1’ and 1) by
optical resolution using lipase-catalyzed enantioselective acylation.
RESULTS AND DISCUSSION
Lipase-catalyzed acetylation of rac-2
We previously reported that synthesis of optically active γ-lactones by lipase-catalyzed kinetic
resolution.11,12 Novozym 435-catalyzed acetylation of racemic N-alkyl-4-hydroxyalkylamides gave both
enantiomers with over 99% optical purities. In this paper, N-alkyl-6-hydroxydodecanamides (2) that
have similar structure to above-mentioned substrates were acetylated using Novozym 435. N-alkyl-6-
hydroxydodecanamide (rac-2a-c) was synthesized by aminolysis of rac-1 with various amines (Scheme
1). The reaction conversion and enantioselectivity by lipase-catalyzed acetylation were investigated
using racemic N-methyl-6-hydroxydodecanamide (rac-2a) as a substrate and various lipases (Table 1).
Vinyl acetate was used as an acyl donor and two solvents, n-hexane and i-Pr2O, were used. Five lipases,
Lipase PS (entries 1-4), Novozym 435 (entries 5 and 6), Lipase AYS (entries 7 and 8), PPL (entries 9 and
10), and Lipozyme RM IM (entries 11 and 12), were used. The reaction hardly progressed at all in
either solvent over three days reaction time with Lipase AYS, PPL, and Lipozyme RM IM (entries 7-12).
The reaction time required to reach 50% conversion was 30 hours when using Lipase PS as a lipase
(entries 1 and 2) and 24 hours using Novozym 435 (entries 5 and 6). This result showed that the
substrate affinity of Novozym 435 to rac-2a was higher than that of Lipase PS. When Novozym 435
was used as a lipase, the acetylation in i-Pr2O was faster than that in n-hexane. However, the
enantiomeric excesses of (R)-N-methyl-6-hydroxydodecanamide [(R)-2a] and (S)-N-methyl-6-
acetoxydodecanamide [(S)-3a] using Novozym 435 were lower than those using Lipase PS. The
enantioselectivity of Lipase PS for rac-2a was higher than that of Novozym 435. The reaction time to
reach 50% conversion required 30 hours in both solvents using Lipase PS (entries 1 and 2). The
enantiomeric excesses of (R)-2a and (S)-3a in n-hexane were higher than those in i-Pr2O. Consequently,
the effect of the R3 group on reaction conversion and enantiomeric excess was confirmed using rac-2a-c,
Lipase PS and n-hexane (entries 1, 3 and 4). Benzyl and isopropyl groups were attempted as R3 as well
as a methyl group. The reaction time required to reach 50% conversion was 30 hours with rac-2a or
rac-2c as a substrate (entries 1 and 3) and 40 hours with rac-2b (entry 4). Therefore, the enantiomeric
excess of (R)-2 and (S)-3 using rac-2a and rac-2c afforded over 90% conversion, and that using rac-2b
afforded about 80% conversion. The substrate affinity of Lipase PS for rac-2b was higher than that for

HETEROCYCLES, Vol. 85, No. 5, 2012
1063
rac-2a and rac-2c. In contrast, the enantioselectivity for rac-2a and rac-2c was higher than for rac-2b.
E value was calculated by using Chen’s equation, and it shows substrate selectivity of enzyme.13 Lipase
PS-catalyzed acetylation of rac-2a and rac-2c in n-hexane gave both enantiomers with over 90% optical
purities, respectively. E value that obtained by enantioselective acetylation of rac-2c was higher than
that of rac-2a (entries 1 and 4). In case of using rac-2a, the highest optical purity was expressed in all
(R)-2. In fact, E value is used as a measurement of enantioselectivity of lipase. Even if E value
showed somewhat low, it was possible to synthesize both enantiomers with over 90% optical purities
(entry 1). Therefore, it was thought that rac-2a with methyl R3 group was suitable for lipase
PS-catalyzed acetylation compared with rac-2c with iso-propyl group.
Novozym 435-catalyzed acetylation of 2a was about 60% enantioselectivity. Lipase PS-catalyzed
acetylation, however, showed over 90% enantioselectivity. It was assumed that the difference of
enantioselectivity was caused by the structure of active site on each lipase. X-Ray crystallography,
which provides important information on the active site of enzymes, has allowed the determination of the
crystal structures of lipases, i.e. human pancreatic lipase, Geotrichum candidum, Candida rugosa and
Pseudomonas glumae.14-17 The active site of Candida antarctica lipase B (Novozym 435) and
Pseudomonas cepacia lipase (Lipase PS), which were used in this investigation, was also reported.18, 19
Active site of lipase, catalytic triad, is generally formed by three amino acids. These are serine, which is
active center, histidine, and aspartate. Since the base sequence of amino acid changes with kinds of
lipase, the shape of activity site also changes with lipase. The active site of Candida antarctica lipase B
is composed by Ser105, His187, and Asp224 and Pseudomonas cepacia lipase is Ser87, His286, and
Asp264.18,19 This base sequence has a huge effect on enantioselectivity of lipase. Kazlauskas et al.
suggested the relationship between the structure of active site and substrate to enantioselectivity.20 An
empirical rule, which is based only on the presence of steric factors in substrate molecules, was proposed
to predict the enantioselectivity toward secondary alcohols and their esters displayed by lipase. Recently,
the relationship between structure of active site and substrate and enantioselectivity of lipase is
reported.21-24 Lemke et al. investigated particularly about the enantioselectivity of Paseudomonas
cepacia lipase using 69 kinds of substrates.25 The kinetic resolution of 3-(aryloxy)propan-2-ol
derivatives by transesterification with vinyl acetate in organic solvents in the presence of Pseudomonas
cepacia lipase was subjected. This lipase showed high E value (>100) to the substrates which have
acyloxy group such as n-pentanoate, n-nonanoate, and n-pentadecanoate. In contrast, it expressed low E
value, about 30, to the similar substrate possessed n-hexanoate, n-octanoate, and n-heptadecanoate. The
slight difference of chain length has a great effect on the enantioselectivity. Furthermore, Lemke et al.
concluded that the enantioselectivity of lipase was determined not by volume of active site but by shape.
Reaction mechanism of lipase-catalyzed acetylation of 2a was shown in Figure 1.26 The reaction of

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HETEROCYCLES, Vol. 85, No. 5, 2012
esterification starts with the formation of tetrahedral intermediate with alcohol, where vinyl acetate is
covalently linked to side-chain oxygen of catalytic serine. The crucial hydrogen bonds from H of
catalytic histidine to serine O and the oxygen of the alcohol moiety are formed. Transfer of H to the
oxygen of the alcohol moiety splits away the alcohol and an acyl enzyme complex is formed, which is
hydrolyzed by the substrate, N-methyl-6-hydroxydodecanamide (2a), to the tetrahedral intermediate with
2a. N-Methyl-6-acetoxydodecanamide (3a) is split from enzyme and free enzyme is formed.
Naoshima et al. explained the enantioselectivity of lipase by computer modeling.27 The hydrolysis of
1-phenylethyl acetate in the presence of Pseudomonas cepacia lipase was investigated, and the
(R)-enantiomer was preferentially hydrolyzed with high enantioselectivity.28 Carbonyl carbon of the
substrate bound the oxygen of Ser87 in Pseudomonas cepacia lipase-catalyzed hydrolysis. It was
proven that the C-O distance of (R)-enantiomer was shorter than that of (S)-enantiomer by computer
modeling. Furthermore, the large difference of C-O distance among each enantiomer gave high
enantioselectivity. The oxygen of hydroxy group in the substrate bound the carbonyl carbon of acetyl
group linked Ser87 in acetylation of 2a. It was assumed that the C-O distance of (S)-enantiomer was
shorter than that of (R)-enantiomer for the shape of active site, and the large difference of C-O distance
gave over 90% enantioselectivity.
1) KOH
MeOH
90 °C
OH
R2
OH
R2
H
N
lipase
HO
R3
2) H+
solvent
MS 4Å
O
R2
O
OAc
lipase
solvent
OH
R2
O
O
H
RNH2
rt
( )-1,1'
R
2a-c 2'a
( )-
,
( )-4,4'
R
R
N
R3
1) K2CO3
MeOH
65 °C
R2
O
O
O
rac
OAc
R2
OH
R2
H
N
H
-1,1'
rac
-2a-c,2'a
N
R3
R
2) H+
3
R2= -C H
,
R = Me: , Bn:
,
O
S
O
S
1 2 3 4
: , ,
13
a
b
c
Pr:
i-
n
6
3a c,3'a
-
2a c,2'a
-
( )-
( )-
1', 2' 3' 4'
-C H :
,
,
n
4
9
2) H+
1) KOH
MeOH
90 °C
OH
R2
lipase
HO
solvent
MS 4Å
R2
O
O
O
( )-1,1'
S
( )-4,4'
S
Scheme 1
Lipase-catalyzed lactonization of rac-4
Generally, γ- and δ-lactones could be synthesized from corresponding hydroxycarboxylic acid under
acidic conditions because they were stable as cyclic esters. However, when the synthesizing medium
and large ring size lactones had more than seven members, the corresponding hydroxycarboxylic acid
reacted under acidic conditions, and inter-molecular esterification had priority over intra-molecular

HETEROCYCLES, Vol. 85, No. 5, 2012
1065
Table 1. Lipase screening in enantioselective acetylation of rac-21)
Yield [%] / Enantiomeric
Excess [% e.e.]2)
Temp. Time
Entry Substrate R3
Lipase
Solvent
E4)
[°C]
[h]
(R)-2
(S)-3
1
2
3
4
rac-2a
Me
Lipase PS n-hexane
i-Pr2O
50
30
30
40
30
44 / 98
45 / 83
51 / 79
50 / 90
52 / 90
49 / 86
49 / 85
50 / 94
87
34
rac-2b
rac-2c
Bn
n-hexane
30
i-Pr
100
Novozym
n-hexane
435
5
rac-2a
Me
60
24
42 / 65
56 / 57
7
6
7
i-Pr2O
Lipase AYS n-hexane
i-Pr2O
33 / 64
67 / 39
2 / n.d.3)
1 / n.d.3)
3 / n.d.3)
trace / n.d.3)
4
-
45
30
72
72
91 / n.d.3)
94 / n.d.3)
96 / n.d.3)
99 / n.d.3)
8
-
9
PPL
n-hexane
i-Pr2O
-
10
-
Lipozyme
RM IM
11
12
n-hexane
i-Pr2O
55
72
91 / n.d.3)
89 / n.d.3)
3 / n.d.3)
7 / n.d.3)
-
-
1) rac-2: 1.0 mmol, vinyl acetate: 2.0 mmol, Lipase PS, Lipase AYS and PPL: 0.5w/w, Novozym 435 and Lipozyme
RM IM: 0.4 g, solvent: 20 mL
2) Determined by GC using InertCap CHIRAMIX column.
3) Not determined
4) E= ln[1-c(1+eep)]/ln[1-c(1-eep)], c= ees/(ees+eep), ee(s)= (R)-2, ee(p): (S)-3
esterification. For this reason, these lactones were generally synthesized by Yamaguchi
macrolactonization, Mukaiyama-Corey macrolactonization, or Shiina macrolactonization.29-31 These
methods required high dilution and complicated processes. We focused on the high molecular
recognition of lipase and hypothesized that lipase produced only ε-dodecalactone from
6-hydroxydodecanoic acid (4). Lipase-catalyzed lactonization of racemic 6-hydroxydodecanoic acid
(rac-4) prepared from ε-dodecalactone (rac-1) by alkaline hydrolysis was investigated using various were
used in this investigation. 1 was not or hardly produced in all solvents despite three days reaction time
using Lipase AYS, and Lipozyme RM IM. In contrast, 1 was produced in all solvents using Novozym
435 (entries 1-3) and the (S)-enantiomer was lactonized preferentially. 1 was obtained with over 50%
yield in three hours reaction time in n-hexane and i-Pr2O. On the other hand, the yield of 1 was 20%

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HETEROCYCLES, Vol. 85, No. 5, 2012
O
O
Ser
Ser
O
O
O
O
H
O
H
H
H
O
N
N
N
N
Asp
Asp
O
O
His
His
free enzyme
tetrahedral intermediate
with alcohol
O
O
H
N
C6H13
O
OH
3a
Ser
Ser
O
O
H
O
O
H
O
N
N
Asp
O
C6H13
O
OH
C6H13
O
H
H
N
N
Asp
His
N
O
O
2a
His
HN
O
tetrahedral intermediate
with 2a
acyl enzyme
Figure 1
despite three days reaction time in THF. This investigation was designed to synthesize both enantiomers
of 1 using the same conditions. In other words, it was designed so that lipase-catalyzed lactonization
progressed with no enantioselectivity and to afford racemic 1 with high yield. Three solvents were used
in this investigation. 1 was synthesized with 30% yield at 40 °C and 3 hours reaction time in Et2O
(entry 4). Lactonization was performed at 80 °C to afford 1 with 79% yield in cyclohexane and CPME
(entries 5 and 6). Additionally, the enantiomeric excess of 1 in CPME was more racemic than that in
cyclohexane. Therefore, entry 9 was determined as the optimum conditions.
Total synthesis of optically active ε-lactones
The results of Lipase PS-catalyzed enantioselective acetylation, alkaline hydrolysis, and Novozym
435-catalyzed lactonization are shown in Table 3. Lipase PS-catalyzed enantioselective acetylation of
rac-2a reached about 50% conversion in 30 hours (entry 1). The conversion was 40% at 30 h using
rac-2’a as a substrate (entry 2). A reaction time of 48 h was required to reach about 50% Conversion
(entry 3). The substrate affinity of Lipase PS to rac-2a was higher than that to rac-2’a.

HETEROCYCLES, Vol. 85, No. 5, 2012
1067
Table 2. Lipase screening in intra-esterification of rac-41)
Temp. Time
Yield [%] / Enantiomeric Excess
[% e.e.]2) / Config.
51 / 60 / (S)
Entry
Lipase
Solvent
[°C]
60
[h]
3
1
2
Novozym 435
n-hexane
i-Pr2O
62 / 29 / (S)
3
THF
72
3
20 / 27 / (S)
4
Et2O
30 / 65 / (S)
5
Cyclohexane
CPME
n-hexane
i-Pr2O
79 / 6 / (S)
6
79 / 3 / (S)
7
Lipase AYS
PPL
45
30
72
72
3 / - / -
8
not produced / - / -
not produced / - / -
not produced / - / -
1 / - / -
9
THF
10
11
12
n-hexane
i-Pr2O
THF
trace / - / -
Lipozyme RM
IM
13
n-hexane
55
72
trace / - / -
14
15
i-Pr2O
trace / - / -
THF
Not produced / - / -
1) rac-4: 1.0 mmol, Lipase AYS and PPL: 0.5w/w, Novozym 435 and Lipozyme RM IM: 0.4 g, solvent: 20
mL, MS 4Å: 1.0 g
2) Determined by GC using InertCap CHIRAMIX column.
Alkaline hydrolysis of 2 and 2’ afforded 4 and 4’ with a midium yield of 70-83%. Both enantiomers of
ε-dodecalactone were successfully synthesized with excellent enantiomeric excesses: (R)-1 and (S)-1 were
98% and 90%, respectively. In the case of lipase-catalyzed acetylation of rac-2’a at 30 hours, (S)-1’
was synthesized with the excellent enantiomeric excess of 92%. (R)-1’ was synthesized at 63%
enantiomeric excess. The acetylation progressed for 48 hours and reached about 50% conversion, and
(S)-1’ and (R)-1’ were produced with 87% and 77% enantiomeric excesses, respectively. The
enantiomeric excess of (R)-1’ increased 14%, while that of (S)-1’ decreased 5%. These results showed
that the (S)-2a’ in the reaction mixture decreased, and it seems that the molecular recognition of Lipase
PS decreased.

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HETEROCYCLES, Vol. 85, No. 5, 2012
Table 3. Synthesis of optically active 1 and 1’
Yield [%] /
Enantiomeric Excess
[% e.e.]1)
Yield
[%]
Yield [%]
Yield [%]
Time
[h]
Entry Substrate
R2
(R)-2 (S)-3
(S)-2
93
(R)-4 (S)-4
(R)-1
(S)-1
1
2
3
rac-2a n-C6H13 30
44
60
51
52
40
48
83
75
70
71
74
77
74 / 98
72 / 63
73 / 77
70 / 90
71 / 92
70 / 87
rac-2’a n-C4H9
30
48
73
71
1) Determined by GC using InertCap CHIRAMIX column.
EXPERIMENTAL
Thin-layer chromatography (TLC) was performed on prepared plates (silica gel F-254 on aluminum;
Merck Ltd., Damstadt, Germany). Crude products were purified by column chromatography on silica
gel FL60D purchased from Fuji Silysia Chemical Ltd. (Aichi, Japan). Melting points (mp) were
recorded on a MP-500D micro-melting-point apparatus from Yanaco Technical Science Co., Ltd. (Kyoto,
Japan) and are uncorrected. Infrared (IR) spectra were recorded on a Fourier transform (FT)
IR-460-plus spectrometer from JASCO Corporation (Tokyo, Japan) and are reported as wave numbers
13
(cm-1). 1H and C NMR spectra were recorded in CDCl3 solution on 500 MHz FT-NMR spectrometer
(JEOL JNM-ECA 500 system). Chemical shifts are reported as parts per million with respect to the
internal tetramethylsilane (TMS). Coupling constants (J) are reported in hertz (Hz). High-resolution
mass spectra (HRMS) were analyzed on a Mariner Biospectrometry Workstation (PerSeptive Biosystems,
Framingham, MA, USA). The enantiomeric excesses were determined using a PerkinElmer Autosystem
XL gas chromatograph equipped with the chiral capillary column InertCap CHIRAMIX (30 m × 0.25 mm
I.D. 0.25 μm film thickness, GC Science Co., Ltd. Tokyo, Japan). The carrier gas was helium and the
flow rate was 2.5 mL/min for ε-Decalactone and ε-dodecalactone or 2.0 mL/min for
N-methyl-6-acetoxydecanamide and N-methyl-6-acetoxydodecanamide.
ε-Decalactone and
ε-dodecalactone were gifts from Soda Aromatic Co., Ltd. (Tokyo, Japan). Novozym 435 (immobilized
lipase from Candida antarctica) and Lipozyme RM IM (immobilized lipase from Rhizomucor miehei)
were gifts from Novozymes A/S (Paraná, Brazil). Lipase PS (from Burkholderia cepacia) and Lipase
AYS (from Candida rugosa) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Porcine pancreatic lipase (PPL) was purchased from Nacalai Tesque Inc. (Kyoto, Japan). All other
materials were commercially obtained. Diethyl ether (Et2O), diisopropyl ether (i-Pr2O), cyclopentyl
methyl ether (CPME), and tetrahydrofuran (THF) were dried before used.

HETEROCYCLES, Vol. 85, No. 5, 2012
1069
Preparation of racemic N-methyl-6-hydroxyalkylamide (rac-2a and rac-2’a)
Racemic N-methyl-6-hydroxyalkylamides (rac-2a and rac-2’a) were prepared by adding methylamine
hydrochloride (1.0 g, 15.0 mmol) and potassium acetate (1.5 g, 15.0 mmol) to a solution of racemic
ε-lactones (10.0 mmol) in THF (30 mL). The mixture was stirred at room temperature. After
evaporation of the corresponding alcohol, H2O was added, and the aqueous phase was extracted with
CHCl3. The combined organic phase was dried over Na2SO4 and concentrated under reduced pressure.
The residue was purified by flash chromatography on silica (eluent: AcOEt) to afford the corresponding
N-methyl-6-hydroxyalkylamides (rac-2a and rac-2a’) as a colorless solid.
Data for racemic N-methyl-6-hydroxydodecanamide (rac-2a)
Colorless solid; mp 74-75 °C; Rf = 0.14 (eluent: AcOEt); IR (KBr, νmax/cm-1): 3305 (-O-H), 3106 (-N-H),
1
2953 (-CH3), 2925 (-CH2-), 2870 (-CH3), 2856 (-CH2-), 1644 (-C(=O)-N-H); H NMR (500 MHz,
CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3, 3H), 1.27-1.53 (m, -CH2- ×7, 14H), 1.59-1.75 (m, -CH2-, 2H),
2.13-2.24 (m, -C(=O)-CH2-, 2H), 2.81 (d, J = 4.6 Hz, -NH-CH3, 3H), 3.57-3.65 (m, -CH-OH, 1H), 5.51
(br s, -NH-, 1H); 13C NMR (126 MHz, CDCl3) δ (ppm): 14.1 (-CH3), 22.6 (-CH2CH3), 25.3 (-CH2-), 25.6
(-CH2-), 26.3 (-NH-CH3), 29.3 (-CH2-), 31.8 (-CH2-), 36.5 (-C(=O)-CH2-), 37.0 (-CH2-), 37.6 (-CH2-),
71.7 (-CH-OH), 173.6 (-NH-C(=O)-); HRMS (ESI) calcd. for C13H28NO2 (M+H)+, 230.2120; found
(M+H)+, 230.2125.
Data for racemic N-methyl-6-hydroxydecanamide (rac-2’a)
Colorless solid; mp 62-63 °C; Rf = 0.14 (eluent: AcOEt); IR (KBr, νmax/cm-1): 3306 (-O-H, -N-H), 2953
1
(-CH3), 2934 (-CH2-), 2869 (-CH3), 2860 (-CH2-), 1644 (-C(=O)-N-H); H NMR (500 MHz, CDCl3) δ
(ppm): 0.90 (t, J = 6.9 Hz, -CH3, 3H), 1.25-1.52 (m, -CH2- ×5, 10H), 1.57-1.75 (m, -CH2-, 2H), 2.19 (t, J
= 7.3 Hz, -C(=O)-CH2-, 2H), 2.80 (d, J = 5.0 Hz, -NH-CH3, 3H), 3.60 (m, -CH-OH, 1H), 5.59 (br s, -NH-,
1H); 13C NMR (126 Hz, CDCl3) δ (ppm): 14.1 (-CH3), 22.7 (-CH2CH3), 25.3 (-CH2-), 25.6 (-CH2-), 26.3
(-NH-CH3), 27.8 (-CH2-), 36.5 (-C(=O)-CH2-), 37.2 (-CH2-), 71.6 (-CH-OH), 173.6 (-NH-C(=O)-);
HRMS (ESI) calcd. for C11H24NO2 (M+H)+, 202.1807; found (M+H)+, 202.1815.
Preparation of racemic N-alkyl-6-hydroxydodecanamide (rac-2b and rac-2c)
Racemic ε-dodecalactone (rac-1) (1.9 g, 10.0 mmol) was added to the corresponding amine (20.0 mmol)
and stirred at room temperature. H2O was added and neutralized with 1 M HCl aq. The aqueous phase
was extracted with CHCl3. The combined organic phase was washed with aqueous NaHCO3 and brine
and dried over Na2SO4. After evaporation of CHCl3, the residue was purified by flash chromatography
on silica (eluent: AcOEt) to afford the corresponding N-alkyl-6-hydroxydodecanamide (rac-2b and
rac-2c) as a colorless solid.
Data for racemic N-benzyl-6-hydroxydodecanamide (rac-2b)
Colorless solid; mp 72-73 °C; Rf = 0.25 (eluent: n-hexane-AcOEt, 1:1, v/v); IR (KBr, νmax/cm-1): 3292

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(-O-H, -N-H), 3087, 3064 (Ar, -C-H), 2956 (-CH3), 2925 (-CH2-), 2873 (-CH3), 2855 (-CH2-), 1634
(-C(=O)-N-H), 1496, 1456 (Ar, -C=C-); 1H NMR (500 MHz, CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3,
3H), 1.28-1.49 (m, -CH2- × 7, 14H), 1.58-1.78 (m, -CH2-, 2H), 2.22 (t, J = 7.3 Hz, -C(=O)-CH2-, 2H),
13
3.57 (m, -CH-OH, 1H), 4.42 (m, -NH-CH2-Ar, 2H), 5.97 (br s, -NH-, 1H), 7.26-7.35 (m, Ar, 5H); C
NMR (126 MHz, CDCl3) δ (ppm): (-CH3), 19.2 (-CH2CH3), 22.6 (-CH2-), 25.2 (-CH2-), 25.5 (-CH2-),
25.6 (-CH2-), 29.3 (-CH2-), 31.8 (-C(=O)-CH2-), 36.5 (-CH2-), 36.9 (-CH2-), 43.5 (-NH-CH2-Ar), 71.6
(-CH-OH), 126.4, 127.4, 127.8, 128.6, 138.4 (Ar), 172.9 (-NH-C(=O)-); HRMS (ESI) calcd. for
C19H32NO2 (M+H)+, 306.2433; found (M+H)+, 306.2442.
Data for racemic N-isopropyl-6-hydroxydodecanamide (rac-2c)
Colorless solid; mp 87-88 °C; Rf = 0.15 (eluent: n-hexane-AcOEt, 1:1, v/v); IR (KBr, νmax/cm-1): 3305
1
(-O-H, -N-H), 2955 (-CH3), 2926 (-CH2-), 2871 (-CH3), 2854 (-CH2-), 1640 (-C(=O)-N-H); H NMR
(500 MHz, CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3, 3H), 1.14 (d, J = 6.4 Hz, -NH-CH(CH3)2, 6H),
1.28-1.51 (m, -CH2- × 7, 14H), 2.09-2.20 (m, -C(=O)-CH2-, 2H), 3.57-3.64 (m, -CH-OH, 1H), 4.08 (m,
-NH-CH(CH3)2, 1H), 5.27 (br s, -NH-, 1H); 13C NMR (126 MHz, CDCl3) δ (ppm): 14.1 (-CH3), 22.6
(-CH2CH3), 22.8 (-NH-CH(CH3)2), 25.2 (-CH2-), 25.6 (-CH2-), 29.3 (-CH2-), 31.8 (-CH2-), 36.8
(-C(=O)-CH2-), 37.0 (-CH2-), 37.6 (-CH2-), 41.2 (-NH-CH(CH3)2), 71.6 (-CH-OH), 172.0 (-NH-C(=O)-);
HRMS (ESI) calcd. for C15H32NO2 (M+H)+, 258.2433; found (M+H)+, 258.2440.
General procedure for lipase-catalyzed acetylation
Lipase (Lipase PS, Lipase AYS and PPL: 0.5 w/w, Novozym 435 and Lipozyme RM IM: 0.4 g) was
added to a solution of rac-2a-c (1.0 mmol) and vinyl acetate (0.2 g, 2.0 mmol) in the solvent (20 mL), the
mixture was stirred. After stirring, the mixture was filtered, and the solvent was evaporated under
reduced pressure. The crude product was purified by flash chromatography on silica (eluent: AcOEt) to
afford the corresponding (R)-2a-c as a colorless solid and (S)-N-alkyl-6-acetoxydodecanamide [(S)-3a-c]
as a colorless oil.
Data for (R)-N-methyl-6-hydroxydodecanamide [(R)-2a]
Colorless solid; [α]D20 -5.0 (98% e.e., c 0.20, MeOH)
Data for (R)-N-benzyl-6-hydroxydodecanamide [(R)-2b]
Colorless solid; [α]D20 -9.3 (79% e.e., c 0.20, MeOH)
Data for (R)-N-isopropyl-6-hydroxydodecanamide [(R)-2c]
Colorless solid; [α]D20 -5.6 (90% e.e., c 0.20, MeOH)
Data for (R)-N-methyl-6-hydroxydecanamide [(R)-2’a]
Colorless solid; [α]D20 +3.2 (77% e.e., c 0.20, MeOH)
Data for (S)-N-methyl-6-acetoxydodecanamide [(S)-3a]
20
Colorless oil; [α]D -22.5 (90% e.e., c 0.20, MeOH); Rf = 0.54 (eluent: AcOEt); IR (NaCl, νmax/cm-1):

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3301 (-N-H), 2933, 2860 (-CH2-), 1737 (-O-C(=O)-), 1650 (-C(=O)-N-H), 1244 (-C-C(=O)-O); 1H NMR
(500 MHz, CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3, 3H), 1.26-1.40 (m, -CH2- × 5, 10H), 1.47-1.57 (m,
-CH2- × 2, 4H), 1.59-1.72 (m, -CH2-, 2H), 2.04 (s, -O-C(=O)-CH3, 3H), 2.17 (t, J = 7.8 Hz, -C(=O)-CH2-,
2H), 2.80 (d, J = 5.0 Hz, -NH-CH3, 3H), 4.85 (quin, J = 6.9 Hz, -CH-O-C(=O)-, 1H), 5.55 (br s, -NH-,
13
1H); C NMR (126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 21.3 (-O-C(=O)-CH3), 22.5 (-CH2CH3), 25.0
(-CH2-), 25.3 (-CH2-), 25.5 (-CH2-), 26.2 (-NH-CH3), 29.1 (-CH2-), 31.7 (-CH2-), 33.8 (-CH2-), 34.1
(-CH2-), 36.5 (-C(=O)-CH2-), 74.1 (-CH-O-C(=O)-), 171.0 (-O-C(=O)-CH3), 173.4 (-NH-C(=O)-);
HRMS (ESI) calcd. for C15H30NO3 (M+H)+, 272.2226; found (M+H)+, 272.2222.
Data for (S)-N-benzyl-6-acetoxydodecanamide [(S)-3b]
20
Colorless oil; [α]D -8.9 (85% e.e., c 0.20, MeOH); Rf = 0.65 (eluent: n-hexane-AcOEt, 1:1, v/v); IR
(NaCl, νmax/cm-1): 3292 (-N-H), 3087, 3064 (Ar, -C-H), 2952 (-CH3), 2930, 2859 (-CH2-), 1735
1
(-O-C(=O)-), 1647 (-C(=O)-N-H), 1496, 1455 (Ar, -C=C-), 1243 (-C-C(=O)-O); H NMR (500 MHz,
CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3, 3H), 1.24-1.41 (m, -CH2- × 5, 10H), 1.47-1.57 (m, -CH2- × 2,
4H), 1.58-1.74 (m, -CH2-, 2H), 2.02 (s, -O-C(=O)-CH3, 3H), 2.20 (t, J = 7.8 Hz, -C(=O)-CH2-, 2H), 4.43
(d, J = 5.6 Hz, -NH-CH2-Ar, 2H), 4.84 (quin, J = 6.8 Hz, -CH-O-C(=O)-, 1H), 5.85 (br s, -NH-, 1H),
13
7.26-7.35 (m, Ar, 5H); C NMR (126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 19.2 (-O-C(=O)-CH3), 21.2
(-CH2CH3), 25.0 (-CH2-), 25.2 (-CH2-), 25.5 (-CH2-), 29.1 (-CH2-), 31.7 (-CH2-), 33.8 (-CH2-), 34.1
(-CH2-), 36.5 (-C(=O)-CH2-), 43.5 (-NH-CH2-Ar), 74.1 (-CH-O-C(=O)-), 127.4, 127.8, 128.7, 138.4 (Ar),
170.9 (-O-C(=O)-CH3), 172.6 (-NH-C(=O)-); HRMS (ESI) calcd. for C21H34NO3 (M+H)+, 348.2539;
found (M+H)+, 348.2543.
Data for (S)-N-isopropyl-6-acetoxydodecanamide [(S)-3c]
20
Colorless oil; [α]D -0.7 (94% e.e., c 0.20, MeOH); Rf = 0.50 (eluent: n-hexane-AcOEt, 1:1, v/v); IR
(NaCl, νmax/cm-1): 3292 (-N-H), 2956 (-CH3), 2932, 2860 (-CH2-), 1738 (-O-C(=O)-O), 1642
(-C(=O)-N-H), 1243 (-C-C(=O)-O); 1H NMR (500 MHz, CDCl3) δ (ppm): 0.88 (t, J = 6.9 Hz, -CH3, 3H),
1.14 (d, J = 6.4 Hz, -NH-CH(CH3)2, 6H), 1.26-1.40 (m, -CH2- × 5, 10H), 1.47-1.57 (m, -CH2- × 2, 4H),
1.58-1.71 (m, -CH2-, 2H), 2.03 (s, -O-C(=O)-CH3, 3H), 2.12 (t, J = 7.3 Hz, -C(=O)-CH2-, 2H), 4.02-4.15
13
(m, -NH-CH(CH3)2, 1H), 4.85 (quin, J = 6.9 Hz, -CH-O-C(=O)-, 1H), 5.37 (br s, -NH-, 1H); C NMR
(126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 21.2 (-O-C(=O)-CH3), 22.5 (-CH2CH3), 22.8 (-NH-CH(CH3)2),
24.9 (-CH2-), 25.2 (-CH2-), 25.6 (-CH2-), 29.1 (-CH2-), 31.7 (-CH2-), 33.8 (-CH2-), 34.1 (-CH2-), 36.7
(-C(=O)-CH2-), 41.2 (-NH-CH(CH3)2), 74.1 (-CH-O-C(=O)-), 170.9 (-O-C(=O)-CH3), 171.8
(-NH-C(=O)-); HRMS (ESI) calcd. for C17H34NO3 (M+H)+, 300.2539; found (M+H)+, 300.2549.
Data for (S)-N-methyl-6-acetoxydecanamide [(S)-3’a]
20
Colorless oil; [α]D -20.5 (87% e.e., c 0.20, MeOH); Rf = 0.43 (eluent: AcOEt); IR (NaCl, νmax/cm-1):
3300 (-N-H), 2953 (-CH3), 2936, 2863 (-CH2-), 1738 (-O-C(=O)-O), 1652 (-C(=O)-N-H), 1243

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(-C-C(=O)-O); 1H NMR (500 MHz, CDCl3) δ (ppm): 0.89 (t, J = 6.9 Hz, -CH3, 3H), 1.17-1.40 (m, -CH2-
× 3, 6H), 1.47-1.57 (m, -CH2- × 2, 4H), 1.59-1.72 (m, -CH2-, 2H), 2.04 (s, -O-C(=O)-CH3, 3H), 2.17 (t, J
= 7.3 Hz, -C(=O)-CH2-, 2H), 2.80 (d, J = 5.0 Hz, -NH-CH3, 3H), 4.85 (quin, J = 6.0 Hz, -CH-O-C(=O)-,
13
1H), 5.55 (br s, -NH-, 1H); C NMR (126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 21.3 (-O-C(=O)-CH3),
22.5 (-CH2CH3), 25.0 (-CH2-), 25.5 (-CH2-), 26.2 (-CH2-), 27.5 (-NH-CH3), 33.7 (-CH2-), 33.8 (-CH2-),
36.5 (-C(=O)-CH2-), 74.1 (-CH-O-C(=O)-), 171.0 (-O-C(=O)-CH3), 173.4 (-NH-C(=O)-); HRMS (ESI)
calcd. for C13H26NO3 (M+H)+, 244.1913; found (M+H)+, 244.1915.
Preparation of racemic 6-hydroxydodecanaoic acid (rac-4)
Racemic 6-hydroxydodecanoic acid (rac-4) was prepared by adding NaOH (3 g) to a solution of
ε-dodecalactone (rac-1) (1.9 g, 10.0 mmol) in MeOH (50 mL). The mixture was stirred at 65 °C for 3 h.
After evaporation of MeOH, H2O was added and neutralized with H3PO4 at 0 °C. The aqueous phase
was extracted with AcOEt. The combined organic phase was washed with brine and dried over Na2SO4,
and concentrated under reduced pressure. The residue was purified by flash chromatography on silica
(eluent: n-hexane-AcOEt, 1:1, v/v) to afford racemic 6-hydroxydodecanoic acid (rac-6) as a colorless
solid (1.6 g, 76%). mp 54-55 °C; IR (KBr, νmax/cm-1): 3242 (-O-H), 2949 (-CH3), 2926 (-CH2-), 2870
(-CH3), 2851 (-CH2-), 1701 (-C=O); 1H NMR (500 MHz, CDCl3) δ (ppm): 0.89 (t, J = 6.9 Hz, -CH3, 3H),
1.29-1.54 (m, -CH2- × 7, 14H), 1.58-1.74 (m, -CH2-, 2H), 2.37 (t, J = 7.3 Hz, -C(=O)-CH2-, 2H),
13
3.58-3.64 (m, -CH-OH, 1H); C NMR (126 MHz, CDCl3) δ (ppm): 14.1 (-CH3), 22.6 (-CH2CH3), 24.6
(-CH2-), 25.1 (-CH2-), 25.6 (-CH2-), 29.3 (-CH2-), 31.8 (-CH2-), 33.9 (-C(=O)-CH2-), 36.9 (-CH2-), 37.5
(-CH2-), 71.8 (-CH-OH), 179.1 (-C(=O)-OH).
General procedure for lipase-catalyzed lactonization
Lipase (Lipase PS, Lipase AYS and PPL: 0.5 w/w, Novozym 435 and Lipozyme RM IM: 0.2 g) and 0.5 g
4Å molecular sieves were added to a solution of rac-4 (0.5 mmol) in solvent (10 mL), and the mixture
was stirred. After stirring, the mixture was filtered, and the solvent was evaporated under reduced
pressure. The crude mixture was purified by flash chromatography on silica (eluent: n-hexane-AcOEt,
4:1, v/v) to afford the corresponding ε-lactones [(R)- and (S)-1 and (R)- and (S)-1’].
Data for (R)-ε-dodecalactone [(R)-1]
20
Colorless oil; [α]D +34.8 (98% e.e., c 0.10, CHCl3); Rf = 0.40 (eluent: n-hexane-AcOEt, 4:1, v/v); IR
(NaCl, νmax/cm-1): 2931, 2860 (-CH2-), 1730 (-C=O), 1254 (-C-C(=O)-O); 1H NMR (500 MHz, CDCl3) δ
(ppm): 0.88 (t, J = 6.4 Hz, -CH3, 3H), 1.28-1.37 (m, -CH2- × 4, 7H), 1.43-1.76 (m, -CH2- × 3, 6H),
1.89-1.96 (m, -CH2- ×2, 3H), 2.57-2.69 (m, -C(=O)-CH2-, 2H), 4.21-4.26 (m, -O-CHCH2-, 1H); 13C NMR
(126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 22.5 (-CH2CH3), 23.0 (-CH2-), 25.3 (-CH2-), 28.2 (-CH2-), 29.0
(-CH2-), 31.6 (-CH2-), 34.5 (-CH2-), 34.9 (-C(=O)-CH2-), 36.3 (-CH2-), 80.5 (-O-CHCH2-), 175.8
(-O-C(=O)-); HRMS (ESI) calcd. for C12H23O2 (M+H)+, 199.1698; found (M+H)+, 199.1692.

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Data for (S)-ε-dodecalactone [(S)-1]
Colorless oil; [α]D20 -31.7 (90% e.e., c 0.10, CHCl3), lit., [α]D -35.1 (98% e.e., c 1.10, CHCl3)32
Data for (R)-ε-decalactone [(R)-1’]
20
Colorless oil; [α]D +14.4 (77% e.e., c 0.10, CHCl3); Rf = 0.41 (eluent: n-hexane-AcOEt, 4:1, v/v); IR
(NaCl, νmax/cm-1): 2935, 2862 (-CH2-), 1730 (-C=O), 1257 (-C-C(=O)-O); 1H NMR (500 MHz, CDCl3) δ
(ppm): 0.91 (t, J = 6.9 Hz, -CH3, 3H), 1.32-1.40 (m, -CH2- × 2, 3H), 1.42-1.76 (m, -CH2- × 3, 6H),
1.90-1.96 (m, -CH2- ×2, 3H), 2.59-2.68 (m, -C(=O)-CH2-, 2H), 4.23-4.28 (m, -O-CHCH2-, 1H); 13C NMR
(126 MHz, CDCl3) δ (ppm): 13.7 (-CH3), 22.2 (-CH2CH3), 22.8 (-CH2-), 27.3 (-CH2-), 28.0 (-CH2-), 34.3
(-CH2-), 34.7 (-C(=O)-CH2-), 35.9 (-CH2-), 80.3 (-O-CHCH2-), 175.6 (-O-C(=O)-); HRMS (ESI) calcd.
for C10H19O2 (M+H)+, 171.1385; found (M+H)+, 171.1381.
Data for (S)-ε-decalactone [(S)-1’]
Colorless oil; [α]D20 -17.1 (92% e.e., c 0.10, CHCl3), lit., [α]D -18.6 (≥98% e.e., c 1.11, CHCl3)33
Preparation of (S)-N-alkyl-6-hydroxyalkylamide [(S)-2a-c and (S)-2’a]
(S)-N-alkyl-6-hydroxyalkylamides [(S)-2a-c and (S)-2’a] were prepared by adding Na2CO3 (1 g) to a
solution of (S)-N-alkyl-6-acetoxyalkylamides in MeOH (20 mL). The mixture was stirred at 65 °C for 3
h. After evaporation of MeOH, H2O was added and the aqueous phase was extracted with AcOEt. The
combined organic phase was washed with brine and dried over Na2SO4 and concentrated under reduced
pressure. The residue was purified by flash chromatography on silica (eluent: AcOEt) to afford (S)-2a-c
and (S)-2’a as a colorless solid with over 90% yield.
Preparation of (R)- and (S)-6-hydroxyalkanoic acid [(R)- and (S)-4, (R)- and (S)-4’]
NaOH (3.0 g) was added to a solution of (R)- and (S)-N-alkyl-6-hydroxyalkylamides [(R)- and (S)-2a-c,
2’a], and the mixture was stirred at 90 °C. After evaporation of MeOH, H2O was added and neutralized
with H3PO4 at 0 °C. The aqueous phase was extracted with AcOEt. The combined organic phase was
washed with brine, dried over Na2SO4, and concentrated under reduced pressure. The residue was
purified by flash chromatography on silica (eluent: n-hexane-AcOEt, 1:1, v/v) to afford the corresponding
6-hydroxyalkanoic acid [(R)- and (S)-4, (R)- and (S)-4’] as a colorless solid.
Data for (R)- and (S)-6-hydroxydodecanoic acid [(R)- and (S)-4]
20
20
Colorless solid; [α]D -34.4 [(R)-enantiomer, 98% e.e., c 0.20, MeOH]; [α]D +20.7 [(S)-enantiomer,
90% e.e., c 0.20, MeOH].
Data for (R)- and (S)-6-hydroxydecanoic acid [(R)- and (S)-4’]
25
25
Colorless solid; mp 29-30 °C; [α]D -27.8 [(R)-enantiomer, 77% e.e., c 0.20, MeOH]; [α]D +47.1
[(S)-enantiomer, 87% e.e., c 0.20, MeOH]; IR (KBr, νmax/cm-1): 3227 (-O-H), 2952 (-CH3), 2926 (-CH2-),
2871 (-CH3), 2858 (-CH2-), 1709 (-C=O); 1H NMR (500 MHz, CDCl3) δ (ppm): 0.91 (t, J = 6.9 Hz, -CH3,
3H), 1.25-1.54 (m, -CH2- × 5, 10H), 1.59-1.72 (m, -CH2-, 2H), 2.36 (t, J = 7.4 Hz, -C(=O)-CH2-, 2H),

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13
3.59-3.65 (m, -CH-OH, 1H); C NMR (126 MHz, CDCl3) δ (ppm): 14.0 (-CH3), 22.7 (-CH2CH3), 24.6
(-CH2-), 25.0 (-CH2-), 27.8 (-CH2-), 33.9 (-C(=O)-CH2-), 36.8 (-CH2-), 37.1 (-CH2-), 71.8 (-CH-OH),
179.2 (-C(=O)-OH).
Chiral GC analyses
Conditions for N-methyl-6-acetoxydodecanamide (3a)
GC Column: InertCap CHIRAMIX (30 m × 0.25 mm × 0.25 μm film thickness). Oven temp.: 160 °C.
tR-(S) enantiomer = 287.2 min, tR-(R) enantiomer = 289.6 min.
Conditions for N-methyl-6-acetoxydecanamide (3a’)
GC Column: InertCap CHIRAMIX (30 m × 0.25 mm × 0.25 μm film thickness). Oven temp.: 160 °C.
tR-(S) enantiomer = 121.1 min, tR-(R) enantiomer = 122.9 min.
Conditions for ε-Dodecalactone (1)
GC Column: InertCap CHIRAMIX (30 m × 0.25 mm × 0.25 μm film thickness). Program: 130-170 °C
at 1.0 °C/min. tR-(R) enantiomer = 34.6 min, tR-(S) enantiomer = 32.0 min.
Conditions for ε-Decalactone (1’)
GC Column: InertCap CHIRAMIX (30 m × 0.25 mm × 0.25 μm film thickness). Program: 120-160 °C
at 1.0 °C/min. tR-(R) enantiome r= 28.1 min, tR-(S) enantiomer = 24.9 min.
ACKNOWLEDGEMENTS
We are grateful to Novozymes A/S for the generous gift of Novozym 435 and Lipozyme RM IM. A
generous gift of ε-decalactone and ε-dodecalactone from Soda Aromatic Co., Ltd. is also acknowledged.
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