Chemical Synthesis of 13C-labelled Monomers for the Solid-Phase and Template Controlled Enzymatic Synthesis of DNA and RNA Oligomers

Article abstract of DOI:10.1016/S0040-4039(00)73458-7

The preparation of 13C-labelled ribonucleosides starting from <13C6>-glucose 1 and the corresponding nucleobases 5a-e or 6a-e (N⁶-benzoyl-adenine, N²-acetyl-guanine, N⁴-benzoyl-cytosine, uracil and thymine) in 47-66percent overall yield is described.Their subsequent transformation into 5'-O-dimethoxytrityl protected DNA-phosphoramidites and 5'-O-dimethoxytrityl-2'-O-trialkylsilyl protected RNA-phosphor-amidites for the solid phase synthesis of DNA- and RNA-oligomers and to 5'-O-ribo- and deoxyribo-nucleosidetriphosphates for template controlled enzymatic synthesis (polymerase- or reverse transcriptase reaction) has been carried out.